DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) (ab210059)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameDoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red)
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Sample typeTissue
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Species reactivityReacts with: Human
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Product overview
Abcam’s DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) (ab210059) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains a HRP-Polymer anti-Mouse IgG, an AP-Polymer anti-Rabbit IgG, and two distinct chromogens. The DAB chromogen (brown color) is used with the HRP-Polymer anti-Mouse IgG and Permanent Red (red color) is used with the AP-Polymer anti-Rabbit IgG. DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.
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Tested applicationsSuitable for: IHCmore details
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 12 ml 36 ml 120 ml Aqueous Mounting Medium 1 x 12ml 2 x 18ml 1 x 120ml DAB Chromogen (20X) 1 x 1.5ml 1 x 2ml 1 x 6ml DAB Substrate 1 x 12ml 2 x 18ml 1 x 120ml Mouse HRP polymer 1 x 6ml 1 x 18ml 1 x 60ml Permanent Red Activator (5X) 1 x 3ml 1 x 7.2ml 2 x 12ml Permanent Red Chromogen (100X) 1 x 150µl 1 x 360µl 1 x 1.2ml Permanent Red Substrate (RTU) 1 x 15ml 2 x 18ml 1 x 120ml Rabbit AP polymer 1 x 6ml 1 x 18ml 1 x 60ml
Applications
Our Abpromise guarantee covers the use of ab210059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC | Use at an assay dependent concentration. |
Protocols
References
This product has been referenced in:
- Zhang YY et al. CD31 regulates metastasis by inducing epithelial-mesenchymal transition in hepatocellular carcinoma via the ITGB1-FAK-Akt signaling pathway. Cancer Lett 429:29-40 (2018). Read more (PubMed: 29746931) »
- Williams AD et al. Human alpha defensin 5 is a candidate biomarker to delineate inflammatory bowel disease. PLoS One 12:e0179710 (2017). Read more (PubMed: 28817680) »
