DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) (ab210059)

Overview

  • Product name
    DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red)
  • Sample type
    Tissue
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) (ab210059) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears.


    Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains a HRP-Polymer anti-Mouse IgG, an AP-Polymer anti-Rabbit IgG, and two distinct chromogens. The DAB chromogen (brown color) is used with the HRP-Polymer anti-Mouse IgG and Permanent Red (red color) is used with the AP-Polymer anti-Rabbit IgG. DoubleStain IHC Kit: M&R on human tissue (DAB & AP/Red) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.

  • Tested applications
    Suitable for: IHCmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 12 ml 36 ml 120 ml
    Aqueous Mounting Medium 1 x 12ml 2 x 18ml 1 x 120ml
    DAB Chromogen (20X) 1 x 1.5ml 1 x 2ml 1 x 6ml
    DAB Substrate 1 x 12ml 2 x 18ml 1 x 120ml
    Mouse HRP polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Permanent Red Activator (5X) 1 x 3ml 1 x 7.2ml 2 x 12ml
    Permanent Red Chromogen (100X) 1 x 150µl 1 x 360µl 1 x 1.2ml
    Permanent Red Substrate (RTU) 1 x 15ml 2 x 18ml 1 x 120ml
    Rabbit AP polymer 1 x 6ml 1 x 18ml 1 x 60ml

Applications

Our Abpromise guarantee covers the use of ab210059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use at an assay dependent concentration.

Protocols

References

This product has been referenced in:
  • Zhang YY  et al. CD31 regulates metastasis by inducing epithelial-mesenchymal transition in hepatocellular carcinoma via the ITGB1-FAK-Akt signaling pathway. Cancer Lett 429:29-40 (2018). Read more (PubMed: 29746931) »
  • Williams AD  et al. Human alpha defensin 5 is a candidate biomarker to delineate inflammatory bowel disease. PLoS One 12:e0179710 (2017). Read more (PubMed: 28817680) »
See all 2 Publications for this product

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