DoubleStain IHC Kit: M&R on human tissue (DAB & FastRed) (ab210062)

Overview

  • Product name
    DoubleStain IHC Kit: M&R on human tissue (DAB & FastRed)
  • Sample type
    Tissue
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s DoubleStain IHC Kit: M&R on human tissue (DAB & FastRed) (ab210062) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears.


    Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains two polymer enzyme conjugates: HRP Polymer anti-Mouse IgG and AP Polymer anti-Rabbit IgG with two distinct substrates/chromogens, DAB (brown color, used with HRP Polymer anti-Mouse IgG) and FastRed (red color, used with AP Polymer anti-Rabbit IgG). DoubleStain IHC Kit: M&R on human tissue (DAB & FastRed) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.

  • Tested applications
    Suitable for: IHCmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 12 ml 36 ml 120 ml
    Aqueous Mounting Medium 1 x 12ml 1 x 36ml 1 x 120ml
    DAB Chromogen (20X) 1 x 1.5ml 1 x 2ml 1 x 6ml
    DAB substrate buffer (RTU) 1 x 12ml 1 x 36ml 1 x 120ml
    Fast Red chromogen 1 x 6 tablets 1 x 18 tablets 1 x 60 tablets
    Fast Red substrate buffer 6 x 5ml 18 x 5ml 60 x 5ml
    Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Rabbit AP polymer 1 x 6ml 1 x 18ml 1 x 60ml

Applications

Our Abpromise guarantee covers the use of ab210062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use at an assay dependent concentration.

Protocols

References

ab210062 has not yet been referenced specifically in any publications.

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