Product nameDoubleStain IHC Kit: M&R on human tissue (DAB & FastRed)
Species reactivityReacts with: Human
Abcam’s DoubleStain IHC Kit: M&R on human tissue (DAB & FastRed) (ab210062) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains two polymer enzyme conjugates: HRP Polymer anti-Mouse IgG and AP Polymer anti-Rabbit IgG with two distinct substrates/chromogens, DAB (brown color, used with HRP Polymer anti-Mouse IgG) and FastRed (red color, used with AP Polymer anti-Rabbit IgG). DoubleStain IHC Kit: M&R on human tissue (DAB & FastRed) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.
Tested applicationsSuitable for: IHCmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 12 ml 36 ml 120 ml Aqueous Mounting Medium 1 x 12ml 1 x 36ml 1 x 120ml DAB Chromogen (20X) 1 x 1.5ml 1 x 2ml 1 x 6ml DAB substrate buffer (RTU) 1 x 12ml 1 x 36ml 1 x 120ml Fast Red chromogen 1 x 6 tablets 1 x 18 tablets 1 x 60 tablets Fast Red substrate buffer 6 x 5ml 18 x 5ml 60 x 5ml Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Rabbit AP polymer 1 x 6ml 1 x 18ml 1 x 60ml
Our Abpromise guarantee covers the use of ab210062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration.|
ab210062 has not yet been referenced specifically in any publications.