DoubleStain IHC Kit: M&R on human tissue (HRP/Green & AP/Red) (ab210061)

Overview

  • Product name

    DoubleStain IHC Kit: M&R on human tissue (HRP/Green & AP/Red)
  • Sample type

    Tissue
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s DoubleStain IHC Kit: M&R on human tissue (HRP/Green & AP/Red) (ab210061) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears.


    Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains a HRP-Polymer anti-Mouse IgG, AP-Polymer anti-Rabbit IgG, and two distinct chromogens, Emerald (green) and Permanent Red (red). User may apply the two enzyme conjugates onto the specimen at the same time and mix them on the slide. Simplified steps offer user much faster and quicker protocol than a sequential procedure. A second advantage is it allows the researcher to visualize when two proteins are co-localized because of the color change when the chromogens overlap that can be semi-quantitative. For example, if the area of co-localization stains blue, the antigen indicated by Emerald is expressed at higher concentration in the cell and if the color is purple, the antigen indicated by Permanent-Red is expressed at higher concentrations DoubleStain IHC Kit: M&R on human tissue (HRP/Green &AP/Red) is a non-biotin system that avoids endogenous biotin non-specific binding.


    The Organic Mounting Medium is not supplied with the 120 mL kit.


    The Permanent Red working solution is not stable. It must be used (freshly) immediately after it is made.

  • Tested applications

    Suitable for: IHCmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 12 ml 36 ml 120 ml
    Emerald Chromogen 1 x 15ml 2 x 18ml 1 x 120ml
    Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Organic Mounting Medium 1 x 12ml 4 x 9ml
    Permanent Red Activator (5X) 1 x 3ml 1 x 7.2ml 2 x 12ml
    Permanent Red Chromogen (100X) 1 x 150µl 1 x 360µl 1 x 1.2ml
    Permanent Red Substrate (RTU) 1 x 15ml 2 x 18ml 1 x 120ml
    Plastic pipette 1 unit 1 unit 1 unit
    Rabbit AP polymer 1 x 6ml 1 x 18ml 1 x 60ml

Applications

Our Abpromise guarantee covers the use of ab210061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use at an assay dependent concentration.

Protocols

References

This product has been referenced in:

  • Mehdi SJ  et al. Mesenchymal stem cells gene signature in high-risk myeloma bone marrow linked to suppression of distinct IGFBP2-expressing small adipocytes. Br J Haematol 184:578-593 (2019). Read more (PubMed: 30408155) »
See 1 Publication for this product

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