• Product name

    DoubleStain IHC Kit: M&Rt on mouse tissue (BCIP & AEC)
  • Assay type

    Polymer Multiplex
  • Product overview

    Abcam's DoubleStain IHC Kit (ab183278) is designed to use with user supplied mouse and rat primary antibody to detect two distinct antigens on mouse tissue or cell samples. This kit has been tested on paraffin embedded tissue, however it can also be used on frozen specimens or freshly prepared monolayer cell smears. This kit is designed not to give background with most mouse strains however there may be some, especially when using frozen tissue, that require additional blocking.

    Double staining is one of most common methods used in immunohistostaining allowing the detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit (ab183278) supplies the user with two polymer enzyme conjugates: Mouse HRP Polymer and Rat AP Polymer and two distinct substrates/chromogens, AEC (Red color, used with the Mouse HRP Polymer) and BCIP/NBT (Purple color, used with the Rat AP Polymer). A Primer step is used to increase specificity of antibody staining. Both enzyme conjugates are applied to the specimen at the same time and mixed on the slide. This kit offers simplified steps making for a quicker and easier protocol than those used in a sequential procedure. Abcam's DoubleStain IHC Kit (ab183278) is a non-biotin system that avoids endogenous biotin non-specific binding.

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details


  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 12 ml 36 ml 120 ml
    AEC Chromogen (20x) 1 x 2ml 1 x 4ml 1 x 12ml
    AEC Substrate (20x) 1 x 1ml 1 x 2ml 1 x 6ml
    Aqueous Mounting Medium 1 x 15ml 2 x 18ml 1 x 120ml
    Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 6ml
    Mouse HRP (AEC) Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Mouse Primer 1 x 12ml 2 x 18ml 1 x 120ml
    MRt Block A 1 x 6ml 1 x 18ml 1 x 60ml
    MRt Block B 1 x 6ml 1 x 18ml 1 x 60ml
    Rat AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Ready to Use BCIP/NBT 1 x 15ml 2 x 18ml 1 x 120ml
  • Research areas


Our Abpromise guarantee covers the use of ab183278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.



ab183278 has not yet been referenced specifically in any publications.

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