Product nameDoubleStain IHC Kit: M&Rt on mouse tissue (BCIP & AEC)
Assay typePolymer Multiplex
Abcam's DoubleStain IHC Kit (ab183278) is designed to use with user supplied mouse and rat primary antibody to detect two distinct antigens on mouse tissue or cell samples. This kit has been tested on paraffin embedded tissue, however it can also be used on frozen specimens or freshly prepared monolayer cell smears. This kit is designed not to give background with most mouse strains however there may be some, especially when using frozen tissue, that require additional blocking.
Double staining is one of most common methods used in immunohistostaining allowing the detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit (ab183278) supplies the user with two polymer enzyme conjugates: Mouse HRP Polymer and Rat AP Polymer and two distinct substrates/chromogens, AEC (Red color, used with the Mouse HRP Polymer) and BCIP/NBT (Purple color, used with the Rat AP Polymer). A Primer step is used to increase specificity of antibody staining. Both enzyme conjugates are applied to the specimen at the same time and mixed on the slide. This kit offers simplified steps making for a quicker and easier protocol than those used in a sequential procedure. Abcam's DoubleStain IHC Kit (ab183278) is a non-biotin system that avoids endogenous biotin non-specific binding.
Tested applicationsSuitable for: IHC-P, IHC-Frmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 12 ml 36 ml 120 ml AEC Chromogen (20x) 1 x 2ml 1 x 4ml 1 x 12ml AEC Substrate (20x) 1 x 1ml 1 x 2ml 1 x 6ml Aqueous Mounting Medium 1 x 15ml 2 x 18ml 1 x 120ml Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 6ml Mouse HRP (AEC) Polymer 1 x 6ml 1 x 18ml 1 x 60ml Mouse Primer 1 x 12ml 2 x 18ml 1 x 120ml MRt Block A 1 x 6ml 1 x 18ml 1 x 60ml MRt Block B 1 x 6ml 1 x 18ml 1 x 60ml Rat AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Ready to Use BCIP/NBT 1 x 15ml 2 x 18ml 1 x 120ml
Our Abpromise guarantee covers the use of ab183278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab183278 has not yet been referenced specifically in any publications.