• Product name

    DoubleStain IHC Kit: M&Rt on mouse tissue (DAB & AP/Red)
  • Assay type

    Polymer Multiplex
  • Product overview

    Abcam's DoubleStain IHC Kit (ab183277) is designed to use with user supplied mouse and rat primary antibody to detect two distinct antigens on mouse tissue or cell samples. This kit can be used on frozen specimens, paraffin–embedded tissues, or freshly prepared monolayer cell smears. This kit is designed not to give background with most mouse strains however there may be some, especially when using frozen tissue, that require additional blocking.


    Double staining is one of most common methods used in immunohistostaining that allows detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit (ab183277) supplies the user with two polymer enzyme conjugates: Mouse HRP Polymer and Rat AP Polymer with two distinct substrates/chromogens, DAB (brown color, used with the Mouse HRP Polymer) and Permanent Red (red color, used with the Rat AP Polymer). A Primer step is used to increase specificity of antibody staining. Both enzyme conjugates are applied to the specimen at the same time and mixed on the slide. This kit offers simplified steps that make for a quicker and easier protocol than those used in a sequential procedure. Abcam's DoubleStain IHC Kit (ab183277) is non-biotin system that avoids endogenous biotin non-specific binding.

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details


  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 12 ml 36 ml 120 ml
    Aqueous Mounting Medium 1 x 15ml 2 x 18ml 1 x 120ml
    Block A 1 x 6ml 1 x 18ml 1 x 60ml
    Block B 1 x 6ml 1 x 18ml 1 x 60ml
    DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml
    DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml
    Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Mouse Primer 1 x 6ml 1 x 18ml 1 x 60ml
    Permanent Red Activator (5x) 1 x 1.4ml 1 x 3.6ml 1 x 12ml
    Permanent Red Chromogen (100x) 1 x 70µl 1 x 180µl 1 x 0.6ml
    Permanent Red Substrate 1 x 7ml 1 x 18ml 1 x 60ml
    Rat AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
  • Research areas


Our Abpromise guarantee covers the use of ab183277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.



ab183277 has not yet been referenced specifically in any publications.

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