Product nameDoubleStain IHC Kit: M&Rt on mouse tissue (DAB & AP/Red)
Assay typePolymer Multiplex
Abcam's DoubleStain IHC Kit (ab183277) is designed to use with user supplied mouse and rat primary antibody to detect two distinct antigens on mouse tissue or cell samples. This kit can be used on frozen specimens, paraffin–embedded tissues, or freshly prepared monolayer cell smears. This kit is designed not to give background with most mouse strains however there may be some, especially when using frozen tissue, that require additional blocking.
Double staining is one of most common methods used in immunohistostaining that allows detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit (ab183277) supplies the user with two polymer enzyme conjugates: Mouse HRP Polymer and Rat AP Polymer with two distinct substrates/chromogens, DAB (brown color, used with the Mouse HRP Polymer) and Permanent Red (red color, used with the Rat AP Polymer). A Primer step is used to increase specificity of antibody staining. Both enzyme conjugates are applied to the specimen at the same time and mixed on the slide. This kit offers simplified steps that make for a quicker and easier protocol than those used in a sequential procedure. Abcam's DoubleStain IHC Kit (ab183277) is non-biotin system that avoids endogenous biotin non-specific binding.
Tested applicationsSuitable for: IHC-P, IHC-Frmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 12 ml 36 ml 120 ml Aqueous Mounting Medium 1 x 15ml 2 x 18ml 1 x 120ml Block A 1 x 6ml 1 x 18ml 1 x 60ml Block B 1 x 6ml 1 x 18ml 1 x 60ml DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml DAB Substrate 1 x 15ml 2 x 18ml 1 x 120ml Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Mouse Primer 1 x 6ml 1 x 18ml 1 x 60ml Permanent Red Activator (5x) 1 x 1.4ml 1 x 3.6ml 1 x 12ml Permanent Red Chromogen (100x) 1 x 70µl 1 x 180µl 1 x 0.6ml Permanent Red Substrate 1 x 7ml 1 x 18ml 1 x 60ml Rat AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
Our Abpromise guarantee covers the use of ab183277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab183277 has not yet been referenced specifically in any publications.