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Abcam's DoubleStain IHC Kit (ab183277) is designed to use with user supplied mouse and rat primary antibody to detect two distinct antigens on mouse tissue or cell samples. This kit can be used on frozen specimens, paraffin–embedded tissues, or freshly prepared monolayer cell smears. This kit is designed not to give background with most mouse strains however there may be some, especially when using frozen tissue, that require additional blocking.
Double staining is one of most common methods used in immunohistostaining that allows detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit (ab183277) supplies the user with two polymer enzyme conjugates: Mouse HRP Polymer and Rat AP Polymer with two distinct substrates/chromogens, DAB (brown color, used with the Mouse HRP Polymer) and Permanent Red (red color, used with the Rat AP Polymer). A Primer step is used to increase specificity of antibody staining. Both enzyme conjugates are applied to the specimen at the same time and mixed on the slide. This kit offers simplified steps that make for a quicker and easier protocol than those used in a sequential procedure. Abcam's DoubleStain IHC Kit (ab183277) is non-biotin system that avoids endogenous biotin non-specific binding.
|Components||12 ml||36 ml||120 ml|
|Aqueous Mounting Medium||1 x 15ml||2 x 18ml||1 x 120ml|
|Block A||1 x 6ml||1 x 18ml||1 x 60ml|
|Block B||1 x 6ml||1 x 18ml||1 x 60ml|
|DAB Chromogen (20x)||1 x 1.5ml||1 x 2ml||1 x 6ml|
|DAB Substrate||1 x 15ml||2 x 18ml||1 x 120ml|
|Mouse HRP Polymer||1 x 6ml||1 x 18ml||1 x 60ml|
|Mouse Primer||1 x 6ml||1 x 18ml||1 x 60ml|
|Permanent Red Activator (5x)||1 x 1.4ml||1 x 3.6ml||1 x 12ml|
|Permanent Red Chromogen (100x)||1 x 70µl||1 x 180µl||1 x 0.6ml|
|Permanent Red Substrate||1 x 7ml||1 x 18ml||1 x 60ml|
|Rat AP Polymer||1 x 6ml||1 x 18ml||1 x 60ml|
Our Abpromise guarantee covers the use of ab183277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab183277 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"