Overview

  • Product name

    DoubleStain IHC Kit: R&Rt on human/mouse tissue (BCIP & AEC)
  • Assay type

    Polymer Multiplex
  • Product overview

    Abcam's Doublestain IHC Kit (ab183284) is designed for use with user supplied rabbit and rat primary antibodies to detect two distinct antigens on human and mouse tissue or cell samples. This kit can be used for frozen or paraffin embedded tissue, or freshly prepared monolayer cell smears.

     

    Double staining is a common method used in immunohistostaining, allowing for the detection of two distinct antigens in a single tissue. Abcam's Doublestain IHC Kit (ab183284) supplies the user with two polymer enzyme conjugates: Rat HRP Polymer (minimal cross reaction to mouse) and Rabbit AP Polymer with two distinct substrates/chromogens, BCIP/NBT and AEC. AEC chromogen reacts with the Rat HRP Polymer conjugate to produce a red color whilst BCIP/NBT reacts with Rabbit AP Polymer to produce a purple color. This kit is a non-biotin system avoiding the extra steps involved in blocking non-specific binding due to endogenous biotin.

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 12 ml 36 ml 120 ml
    AEC Chromogen (20x) 1 x 2ml 1 x 4ml 1 x 12ml
    AEC Substrate (20x) 1 x 1ml 1 x 2ml 1 x 6ml
    Aqueous Mounting Medium 1 x 15ml 2 x 18ml 1 x 120ml
    Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 6ml
    Rabbit AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Rat HRP (AEC) Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Ready to Use BCIP/NBT 1 x 15ml 2 x 18ml 1 x 120ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Protocols

References

ab183284 has not yet been referenced specifically in any publications.

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