Product nameDoubleStain IHC Kit: R&Rt on human/mouse tissue (DAB & AP/Red)
Assay typePolymer Multiplex
Abcam's Doublestain IHC Kit (ab183283) is designed for use with user supplied rabbit and rat primary antibodies to detect two distinct antigens on human and mouse tissue or cell samples. This kit has been tested on paraffin embedded tissue, however, it can also be used to stain frozen specimens and freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistostaining, allowing for the detection of two distinct antigens in a single tissue. This kit supplies the user with two polymer enzyme conjugates: Rat HRP polymer (minimal cross reaction to mouse) and Rabbit AP polymer with two distinct substrates/chromogens, DAB and Permanent Red. DAB chromogen reacts with the Rat HRP Polymer to produce a brown color. Permanent Red reacts with Rabbit AP Polymer to produce a red color. Abcam's Doublestain IHC Kit (ab183283) is a non-biotin system avoiding the extra steps involved in blocking non-specific binding due to endogenous biotin.
Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 12 ml 36 ml 120 ml Aqueous Mounting Medium 1 x 15ml 2 x 18ml 1 x 120ml DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml DAB Substrate 1 x 12ml 2 x 18ml 1 x 120ml Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 2 x 12ml Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 1.2ml Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 120ml Rabbit AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Rat HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
Our Abpromise guarantee covers the use of ab183283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
ab183283 has not yet been referenced specifically in any publications.