Key features and details
- Rabbit polyclonal to DP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DP1 antibody
See all DP1 primary antibodies
DescriptionRabbit polyclonal to DP1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human DP1 aa 1-290 (N terminal).
Database link: Q14186
- WB: LO2 and Raji cell lysates, mouse kidney tissue lysate. IHC: human lung and human colon damage tissues.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 45 kDa.|
FunctionCan stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1/DP complex appears to mediate both cell proliferation and apoptosis.
Tissue specificityHighest levels in muscle. Also expressed in brain, placenta, liver and kidney. Lower levels in lung and pancreas. Not detected in heart.
Sequence similaritiesBelongs to the E2F/DP family.
modificationsPhosphorylation by E2F-1-bound cyclin A-CDK2, in the S phase, inhibits E2F-mediated DNA binding and transactivation.
- Information by UniProt
- DP 1 antibody
- DP1 antibody
- DRTF1 antibody
Paraffin-embedded human lung tissue stained for DP1 using ab186831 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-DP1 antibody (ab186831) at 1/3000 dilution
Lane 1 : LO2 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Mouse kidney tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 90 seconds
Blocking buffer: 3% nonfat dry milk in TBST.
Paraffin-embedded human colon damage stained for DP1 using ab186831 at 1/200 dilution in immunohistochemical analysis.
ab186831 has not yet been referenced specifically in any publications.