Key features and details
- Rabbit polyclonal to DP2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DP2 antibody
See all DP2 primary antibodies
DescriptionRabbit polyclonal to DP2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human DP2 aa 344-386.
Database link: Q14188
- WB: Mouse heart lysate; HeLa whole cell lysate. IHC-P: Human gastric cancer and kidney tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 49 kDa.|
FunctionCan stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1/DP complex appears to mediate both cell proliferation and apoptosis.
Tissue specificityHigh levels in heart and skeletal muscle. Also found in placenta, kidney, brain, lung and liver. The presence as well as the abundance of the different transcripts appear to vary significantly in different tissues and cell lines.
Sequence similaritiesBelongs to the E2F/DP family.
modificationsSer-24 is probably phosphorylated by CDK2.
- Information by UniProt
- DP2 antibody
- E2F dimerization partner 2 antibody
- Tfdp2 antibody
All lanes : Anti-DP2 antibody (ab235830) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000000 dilution
Predicted band size: 49 kDa
Paraffin-embedded human gastric cancer tissue stained for DP2 using ab235830 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for DP2 using ab235830 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235830 has not yet been referenced specifically in any publications.