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    dpd-antibody-ab180609.pdf

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Signal Transduction Metabolism Amino Acids
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Anti-DPD antibody (ab180609)

  • Datasheet
  • SDS
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Western blot - Anti-DPD antibody (ab180609)

    Key features and details

    • Rabbit polyclonal to DPD
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human DPD protein (ab114584)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-DPD antibody
      See all DPD primary antibodies
    • Description

      Rabbit polyclonal to DPD
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Human
    • Immunogen

      Recombinant full length protein corresponding to Human DPD aa 1 to the C-terminus.
      Database link: Q12882-2

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: THP-1 cell lysate. Mouse liver tissue lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 49% PBS, 50% Glycerol
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Amino Acids
      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Amino acid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)
      • THP-1 whole cell lysate (ab7913)
    • Recombinant Protein

      • Recombinant Human DPD protein (ab114584)
    • Related Products

      • Recombinant Human DPD protein (ab114584)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab180609 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/2000. Predicted molecular weight: 19, 111 kDa.
    Notes
    WB
    1/500 - 1/2000. Predicted molecular weight: 19, 111 kDa.

    Target

    • Function

      Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil.
    • Tissue specificity

      Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.
    • Pathway

      Amino-acid biosynthesis; beta-alanine biosynthesis.
    • Involvement in disease

      Defects in DPYD are the cause of dihydropyrimidine dehydrogenase deficiency (DPYD deficiency) [MIM:274270]; also known as hereditary thymine-uraciluria or familial pyrimidinemia. DPYD deficiency is a disease characterized by persistent urinary excretion of excessive amounts of uracil, thymine and 5-hydroxymethyluracil. Patients suffering from this disease show a severe reaction to the anticancer drug 5-fluorouracil. This reaction includes stomatitis, Leukopenia, thrombocytopenia, hair loss, diarrhea, fever, marked weight loss, cerebellar ataxia, and neurologic symptoms, progressing to semicoma.
    • Sequence similarities

      Belongs to the dihydropyrimidine dehydrogenase family.
      Contains 3 4Fe-4S ferredoxin-type domains.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q12882 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1806 Human
      • Entrez Gene: 99586 Mouse
      • GenBank: AAH08379 Human
      • Omim: 274270 Human
      • Omim: 612779 Human
      • SwissProt: Q12882 Human
      • SwissProt: Q8CHR6 Mouse
      • Unigene: 335034 Human
      • Unigene: 27907 Mouse
      see all
    • Alternative names

      • DHP antibody
      • DHPDHase antibody
      • Dihydropyrimidine dehydrogenase [NADP(+)] antibody
      • Dihydropyrimidine dehydrogenase [NADP+] antibody
      • Dihydropyrimidine dehydrogenase antibody
      • Dihydrothymine dehydrogenase antibody
      • Dihydrouracil dehydrogenase antibody
      • DPD antibody
      • DPYD antibody
      • DPYD_HUMAN antibody
      • MGC132008 antibody
      • MGC70799 antibody
      • OTTHUMP00000058954 antibody
      see all

    Images

    • Western blot - Anti-DPD antibody (ab180609)
      Western blot - Anti-DPD antibody (ab180609)
      All lanes : Anti-DPD antibody (ab180609) at 1/1000 dilution

      Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell lysate
      Lane 2 : Mouse Liver tissue lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 19, 111 kDa
      Observed band size: 112 kDa why is the actual band size different from the predicted?



      Blocking buffer: 3% non-fat dry milk in TBST.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab180609? Please let us know so that we can cite the reference in this datasheet.

    ab180609 has been referenced in 1 publication.

    • Lin K  et al. Long noncoding RNA LINC00261 induces chemosensitization to 5-fluorouracil by mediating methylation-dependent repression of DPYD in human esophageal cancer. FASEB J 33:1972-1988 (2019). PubMed: 30226808

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