Anti-DPD antibody (ab180609)
Key features and details
- Rabbit polyclonal to DPD
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DPD antibody
See all DPD primary antibodies -
Description
Rabbit polyclonal to DPD -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human DPD aa 1 to the C-terminus.
Database link: Q12882-2 -
Positive control
- WB: THP-1 cell lysate. Mouse liver tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 19, 111 kDa.
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| Notes |
|---|
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WB
1/500 - 1/2000. Predicted molecular weight: 19, 111 kDa. |
Target
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Function
Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. -
Tissue specificity
Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. -
Pathway
Amino-acid biosynthesis; beta-alanine biosynthesis. -
Involvement in disease
Defects in DPYD are the cause of dihydropyrimidine dehydrogenase deficiency (DPYD deficiency) [MIM:274270]; also known as hereditary thymine-uraciluria or familial pyrimidinemia. DPYD deficiency is a disease characterized by persistent urinary excretion of excessive amounts of uracil, thymine and 5-hydroxymethyluracil. Patients suffering from this disease show a severe reaction to the anticancer drug 5-fluorouracil. This reaction includes stomatitis, Leukopenia, thrombocytopenia, hair loss, diarrhea, fever, marked weight loss, cerebellar ataxia, and neurologic symptoms, progressing to semicoma. -
Sequence similarities
Belongs to the dihydropyrimidine dehydrogenase family.
Contains 3 4Fe-4S ferredoxin-type domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1806 Human
- Entrez Gene: 99586 Mouse
- GenBank: AAH08379 Human
- Omim: 274270 Human
- Omim: 612779 Human
- SwissProt: Q12882 Human
- SwissProt: Q8CHR6 Mouse
- Unigene: 335034 Human
see all -
Alternative names
- DHP antibody
- DHPDHase antibody
- Dihydropyrimidine dehydrogenase [NADP(+)] antibody
see all
Images
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Western blot - Anti-DPD antibody (ab180609)All lanes : Anti-DPD antibody (ab180609) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 2 : Mouse Liver tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 19, 111 kDa
Observed band size: 112 kDa why is the actual band size different from the predicted?Blocking buffer: 3% non-fat dry milk in TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab180609 has been referenced in 1 publication.
- Lin K et al. Long noncoding RNA LINC00261 induces chemosensitization to 5-fluorouracil by mediating methylation-dependent repression of DPYD in human esophageal cancer. FASEB J 33:1972-1988 (2019). PubMed: 30226808