Recombinant
RabMAb

Recombinant Anti-DPD antibody [EPR8811] - BSA and Azide free (ab231701)

Overview

  • Product name

    Anti-DPD antibody [EPR8811] - BSA and Azide free
    See all DPD primary antibodies
  • Description

    Rabbit monoclonal [EPR8811] to DPD - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DPD aa 850-950. The exact sequence is proprietary.

  • Positive control

    • ICC/IF: HeLa cells. IHC-P: Human colon and lung tissue.
  • General notes

    ab231701 is the carrier-free version of ab134922 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab231701 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab231701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil.
    • Tissue specificity

      Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.
    • Pathway

      Amino-acid biosynthesis; beta-alanine biosynthesis.
    • Involvement in disease

      Defects in DPYD are the cause of dihydropyrimidine dehydrogenase deficiency (DPYD deficiency) [MIM:274270]; also known as hereditary thymine-uraciluria or familial pyrimidinemia. DPYD deficiency is a disease characterized by persistent urinary excretion of excessive amounts of uracil, thymine and 5-hydroxymethyluracil. Patients suffering from this disease show a severe reaction to the anticancer drug 5-fluorouracil. This reaction includes stomatitis, Leukopenia, thrombocytopenia, hair loss, diarrhea, fever, marked weight loss, cerebellar ataxia, and neurologic symptoms, progressing to semicoma.
    • Sequence similarities

      Belongs to the dihydropyrimidine dehydrogenase family.
      Contains 3 4Fe-4S ferredoxin-type domains.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • DHP antibody
      • DHPDHase antibody
      • Dihydropyrimidine dehydrogenase [NADP(+)] antibody
      • Dihydropyrimidine dehydrogenase [NADP+] antibody
      • Dihydropyrimidine dehydrogenase antibody
      • Dihydrothymine dehydrogenase antibody
      • Dihydrouracil dehydrogenase antibody
      • DPD antibody
      • DPYD antibody
      • DPYD_HUMAN antibody
      • MGC132008 antibody
      • MGC70799 antibody
      • OTTHUMP00000058954 antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis Jurkat (human acute T cell leukaemia) cells labelling DPD with purified ab134922 at a dilution of 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at dilution of 1/1000 was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134922)

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue staining DPD with ab134922 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134922)

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human lung tissue staining DPD with ab134922 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134922)

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab231701 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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