Overview

  • Product name

    Anti-DPEP1/MDP antibody [EPR22222]
    See all DPEP1/MDP primary antibodies
  • Description

    Rabbit monoclonal [EPR22222] to DPEP1/MDP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DPEP1/MDP aa 300-400. The exact sequence is proprietary.
    Database link: P16444

  • Positive control

    • WB: Human kidney and testis lysates. IHC-P: Human kidney, colon cancer and testis tissue.
  • General notes

     This product was previously labelled as DPEP1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab230977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 46 kDa).
IHC-P 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity.
    • Sequence similarities

      Belongs to the peptidase M19 family.
    • Cellular localization

      Apical cell membrane. Cell projection > microvillus membrane. Brush border membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Dehydropeptidase-I antibody
      • Dipeptidase 1 (renal) antibody
      • Dipeptidase 1 antibody
      • DPEP1 antibody
      • DPEP1_HUMAN antibody
      • hRDP antibody
      • MBD1 antibody
      • MDP antibody
      • Microsomal dipeptidase antibody
      • RDP antibody
      • Renal dipeptidase antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling DPEP1/MDP with ab230977 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in apical surface of human colon cancer (PMID: 28413640; PMID 23839495) is observed. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DPEP1/MDP with ab230977 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the basement membrane of human testis is observed. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling DPEP1/MDP with ab230977 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proximal tubules of human kidney (PMID: 8663190) is observed. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • All lanes : Anti-DPEP1/MDP antibody [EPR22222] (ab230977) at 1/1000 dilution

      Lane 1 : Human kidney lysate
      Lane 2 : Human testis lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 46 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?


      Exposure time: 48 seconds


      This blot was developed using a higher sensitivity ECL substrate.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 23839495).

    References

    ab230977 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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