Recombinant Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

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Product name

Anti-DPEP1/MDP antibody [EPR22225]
See all DPEP1/MDP primary antibodies
Description

Rabbit monoclonal [EPR22225] to DPEP1/MDP
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment within Human DPEP1/MDP aa 1-400. The exact sequence is proprietary.
Database link: P16444
Positive control
- WB: Human kidney and testis lysates. IHC-P: Human kidney, colon cancer and testis tissue.
General notes

This product was previously labelled as DPEP1

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22225
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- Other

Alternative Versions
- Anti-DPEP1/MDP antibody [EPR22225] - BSA and Azide free (ab242084)
Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
Immunohistochemistry reagents
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Our Abpromise guarantee covers the use of ab230978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 46 kDa).
IHC-P		1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt,ICC/IF or IP.

Function

Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity.
Sequence similarities

Belongs to the peptidase M19 family.
Cellular localization

Apical cell membrane. Cell projection > microvillus membrane. Brush border membrane.
Target information above from: UniProt accession P16444 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1800 Human
- Omim: 179780 Human
- SwissProt: P16444 Human
- Unigene: 109 Human
Alternative names
- Dehydropeptidase-I antibody
- Dipeptidase 1 (renal) antibody
- Dipeptidase 1 antibody
- DPEP1 antibody
- DPEP1_HUMAN antibody
- hRDP antibody
- MBD1 antibody
- MDP antibody
- Microsomal dipeptidase antibody
- RDP antibody
- Renal dipeptidase antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling DPEP1/MDP with ab230978 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in apical surface of human colon cancer (PMID: 28413640; PMID 23839495) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DPEP1/MDP with ab230978 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in basement membrane of human testis is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling DPEP1/MDP with ab230978 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proximal tubules of human kidney (PMID: 8663190) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

All lanes : Anti-DPEP1/MDP antibody [EPR22225] (ab230978) at 1/1000 dilution

Lane 1 : Human kidney lysate at 20 µg
Lane 2 : Human testis lysate at 10 µg

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 46 kDa
Observed band size: 55 kDa
why is the actual band size different from the predicted?

Exposure time: 50 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 23839495).

Datasheet

ab230978 has not yet been referenced specifically in any publications.

Publishing research using ab230978? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Immunohistochemistry reagents

Isotype control

Applications

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Datasheets and documents

References

Customer reviews and Q&As