RecombinantRabMAb

Recombinant Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
  • Western blot - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
  • Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22225] to DPEP1/MDP
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-DPEP1/MDP antibody [EPR22225]
    See all DPEP1/MDP primary antibodies

  • Description

    Rabbit monoclonal [EPR22225] to DPEP1/MDP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human DPEP1/MDP aa 1-400. The exact sequence is proprietary.
    Database link: P16444

  • Positive control

    • WB: Human kidney and testis lysates. IHC-P: Human kidney, colon cancer and testis tissue.

  • General notes

     This product was previously labelled as DPEP1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22225

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 46 kDa).
IHC-P 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity.

    • Sequence similarities

      Belongs to the peptidase M19 family.

    • Cellular localization

      Apical cell membrane. Cell projection > microvillus membrane. Brush border membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Dehydropeptidase-I antibody
        • Dipeptidase 1 (renal) antibody
        • Dipeptidase 1 antibody
        • DPEP1 antibody
        • DPEP1_HUMAN antibody
        • hRDP antibody
        • MBD1 antibody
        • MDP antibody
        • Microsomal dipeptidase antibody
        • RDP antibody
        • Renal dipeptidase antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

        Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling DPEP1/MDP with ab230978 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in apical surface of human colon cancer (PMID: 28413640; PMID 23839495) is observed. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
        Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

        Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DPEP1/MDP with ab230978 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in basement membrane of human testis is observed. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
        Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

        Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling DPEP1/MDP with ab230978 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in proximal tubules of human kidney (PMID: 8663190) is observed. Counterstained with hematoxylin.
        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
        Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      • Western blot - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
        Western blot - Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
        All lanes : Anti-DPEP1/MDP antibody [EPR22225] (ab230978) at 1/1000 dilution

        Lane 1 : Human kidney lysate at 20 µg
        Lane 2 : Human testis lysate at 10 µg

        Secondary
        All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

        Predicted band size: 46 kDa
        Observed band size: 55 kDa
        why is the actual band size different from the predicted?


        Exposure time: 50 seconds


        Blocking/Dilution buffer: 5% NFDM/TBST.

        The molecular weight observed is consistent with what has been described in the literature (PMID: 23839495).

      • Anti-DPEP1/MDP antibody [EPR22225] (ab230978)
        Anti-DPEP1/MDP antibody [EPR22225] (ab230978)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab230978? Please let us know so that we can cite the reference in this datasheet.

      ab230978 has not yet been referenced specifically in any publications.

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