Recombinant
RabMAb

Recombinant Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (ab238646)

Overview

  • Product name

    Anti-DPEP3 antibody [EPR21862] - BSA and Azide free
    See all DPEP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR21862] to DPEP3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DPEP3 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9H4B8

  • Positive control

    • IHC-P: Human testis tissue.
  • General notes

    Ab238646 is the carrier-free version of ab235451. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238646 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 54 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Probable metalloprotease which hydrolyzes cystinyl-bis-glycine. May be involved in meiosis.
  • Sequence similarities

    Belongs to the peptidase M19 family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dipeptidase 3 antibody
    • Dpep3 antibody
    • DPEP3_HUMAN antibody
    • MBD3 antibody
    • membrane bound dipeptidase 3 antibody
    • metallopeptidase (family M19) antibody
    see all

Images

  • DPEP3 was immunoprecipitated from 0.35 mg of human testis lysate with ab235451 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235451 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human testis lysate 10 μg (Input).
    Lane 2: ab235451 IP in human testis lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235451 in human testis lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235451).

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling DPEP3 with ab235451 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on tumor epithelial cells of one case human breast cancer is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Please note that DPEP3 was not detected in every breast cancer tissue sample tested due to the heterogeneity of expression.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235451).

  • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DPEP3 with ab235451 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic staining on human germ cells but not Leydig cells (arrow) or Sertoli cells (arrowhead) (PMID: 21724266) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235451).

     

References

ab238646 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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