Overview

  • Product name
  • Description
    Rabbit polyclonal to DPF2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 125-175 of Human DPF2 (NP_006259.1).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab128149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the requiem/DPF family.
    Contains 1 C2H2-type zinc finger.
    Contains 2 PHD-type zinc fingers.
  • Cellular localization
    Nucleus. Cytoplasm. 30% nuclear. 70% cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis response zinc finger protein antibody
    • BAF45D antibody
    • BRG1-associated factor 45D antibody
    • D4 antibody
    • D4 zinc and double PHD fingers family 2 antibody
    • Double PHD fingers 2 antibody
    • double PHD fingers family 2 antibody
    • DPF 2 antibody
    • DPF2 antibody
    • MGC10180 antibody
    • Protein requiem antibody
    • REQ antibody
    • REQU_HUMAN antibody
    • Requiem antibody
    • Requiem apoptosis response zinc finger antibody
    • UBI D4 antibody
    • ubi-d4 antibody
    • UBID 4 antibody
    • UBID4 antibody
    • zinc and double PHD fingers family 2 antibody
    • Zinc finger protein ubi d4 antibody
    • Zinc finger protein ubi-d4 antibody
    see all

Images

  • Anti-DPF2 antibody (ab128149) at 1 µg/ml + HeLa whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 44 kDa


    Exposure time: 30 seconds
  • Detection of DPF2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab128149 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab128149 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
ChIP
Sample
Human Cell lysate - nuclear (Jurkat Cells)
Negative control
Comparing to input
Specification
Jurkat Cells
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
ab134942, which has worked in the past
Username

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Verified customer

Submitted Dec 11 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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