Anti-DPF2 antibody [EPR9206(B)] - BSA and Azide free (ab232327)

Overview

  • Product name
    Anti-DPF2 antibody [EPR9206(B)] - BSA and Azide free
    See all DPF2 primary antibodies
  • Description
    Rabbit monoclonal [EPR9206(B)] to DPF2 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DPF2 aa 50-150. The exact sequence is proprietary.
    Database link: Q92785

  • Positive control
    • IHC-P: Human colon tissue.
  • General notes

    ab232327 is a PBS-only buffer format of ab134942. Please refer to ab134942 for recommended dilutions, protocols, and image data.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab232327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the requiem/DPF family.
    Contains 1 C2H2-type zinc finger.
    Contains 2 PHD-type zinc fingers.
  • Cellular localization
    Nucleus. Cytoplasm. 30% nuclear. 70% cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis response zinc finger protein antibody
    • BAF45D antibody
    • BRG1-associated factor 45D antibody
    • D4 antibody
    • D4 zinc and double PHD fingers family 2 antibody
    • Double PHD fingers 2 antibody
    • double PHD fingers family 2 antibody
    • DPF 2 antibody
    • DPF2 antibody
    • MGC10180 antibody
    • Protein requiem antibody
    • REQ antibody
    • REQU_HUMAN antibody
    • Requiem antibody
    • Requiem apoptosis response zinc finger antibody
    • UBI D4 antibody
    • ubi-d4 antibody
    • UBID 4 antibody
    • UBID4 antibody
    • zinc and double PHD fingers family 2 antibody
    • Zinc finger protein ubi d4 antibody
    • Zinc finger protein ubi-d4 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling DPF2 with purified ab134942 at 1/200 dilution (8.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9. Tissue was counterstained with hematoxylin. ab97051, a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134942).

References

ab232327 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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