Key features and details
- Mouse monoclonal [2283C1a] to DPF2/REQ
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-DPF2/REQ antibody [2283C1a]
See all DPF2/REQ primary antibodies
DescriptionMouse monoclonal [2283C1a] to DPF2/REQ
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human DPF2/REQ (internal sequence).
This product was previously labelled as DPF2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesab74519 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and then filtered through a 0.22µm membrane.
Our Abpromise guarantee covers the use of ab74519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 44 kDa.|
|Dot blot||Use at an assay dependent dilution.|
FunctionMay be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
Sequence similaritiesBelongs to the requiem/DPF family.
Contains 1 C2H2-type zinc finger.
Contains 2 PHD-type zinc fingers.
Cellular localizationNucleus. Cytoplasm. 30% nuclear. 70% cytoplasmic.
- Information by UniProt
- Apoptosis response zinc finger protein antibody
- BAF45D antibody
- BRG1-associated factor 45D antibody
Anti-DPF2/REQ antibody [2283C1a] (ab74519) + Immunising recombinant protein
Predicted band size: 44 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
ab74519 has not yet been referenced specifically in any publications.