Key features and details
- Rabbit polyclonal to DPF2/REQ
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DPF2/REQ antibody
See all DPF2/REQ primary antibodies
DescriptionRabbit polyclonal to DPF2/REQ
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to Human DPF2/REQ aa 125-175.
Database link: NP_006259.1
- HeLa whole cell lysate
This product was previously labelled as DPF2
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab128149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 44 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
Sequence similaritiesBelongs to the requiem/DPF family.
Contains 1 C2H2-type zinc finger.
Contains 2 PHD-type zinc fingers.
Cellular localizationNucleus. Cytoplasm. 30% nuclear. 70% cytoplasmic.
- Information by UniProt
- Apoptosis response zinc finger protein antibody
- BAF45D antibody
- BRG1-associated factor 45D antibody
Anti-DPF2/REQ antibody (ab128149) at 1 µg/ml + HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 30 seconds
Detection of DPF2/REQ in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab128149 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab128149 has not yet been referenced specifically in any publications.