Overview

  • Product name

  • Description

    Rabbit polyclonal to DPF2/REQ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human DPF2/REQ aa 125-175.
    Database link: NP_006259.1

  • Positive control

    • HeLa whole cell lysate
  • General notes

     This product was previously labelled as DPF2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab128149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the requiem/DPF family.
    Contains 1 C2H2-type zinc finger.
    Contains 2 PHD-type zinc fingers.
  • Cellular localization

    Nucleus. Cytoplasm. 30% nuclear. 70% cytoplasmic.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis response zinc finger protein antibody
    • BAF45D antibody
    • BRG1-associated factor 45D antibody
    • D4 antibody
    • D4 zinc and double PHD fingers family 2 antibody
    • Double PHD fingers 2 antibody
    • double PHD fingers family 2 antibody
    • DPF 2 antibody
    • DPF2 antibody
    • MGC10180 antibody
    • Protein requiem antibody
    • REQ antibody
    • REQU_HUMAN antibody
    • Requiem antibody
    • Requiem apoptosis response zinc finger antibody
    • UBI D4 antibody
    • ubi-d4 antibody
    • UBID 4 antibody
    • UBID4 antibody
    • zinc and double PHD fingers family 2 antibody
    • Zinc finger protein ubi d4 antibody
    • Zinc finger protein ubi-d4 antibody
    see all

Images

  • Anti-DPF2/REQ antibody (ab128149) at 1 µg/ml + HeLa whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 44 kDa


    Exposure time: 30 seconds
  • Detection of DPF2/REQ in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab128149 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab128149 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
ChIP
Sample
Human Cell lysate - nuclear (Jurkat Cells)
Negative control
Comparing to input
Specification
Jurkat Cells
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
ab134942, which has worked in the past

Abcam user community

Verified customer

Submitted Dec 11 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up