Key features and details
- Rabbit polyclonal to DPF2/REQ
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DPF2/REQ antibody
See all DPF2/REQ primary antibodies
DescriptionRabbit polyclonal to DPF2/REQ
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Chimpanzee
Recombinant fragment within Human DPF2/REQ (internal sequence). The exact sequence is proprietary.
Database link: Q92785
- IHC-P: H661 xenograft tissue; Mouse brain tissue. WB: DPF2/REQ transfected HEK-293T cell lysate.
This product was previously labelled as DPF2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 44 kDa.|
FunctionMay be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
Sequence similaritiesBelongs to the requiem/DPF family.
Contains 1 C2H2-type zinc finger.
Contains 2 PHD-type zinc fingers.
Cellular localizationNucleus. Cytoplasm. 30% nuclear. 70% cytoplasmic.
- Information by UniProt
- Apoptosis response zinc finger protein antibody
- BAF45D antibody
- BRG1-associated factor 45D antibody
Paraffin-embedded mouse brain tissue stained for DPF2/REQ using ab227095 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-DPF2/REQ antibody (ab227095) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : DPF2/REQ transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 44 kDa
12% SDS-PAGE gel.
Paraffin-embedded H661 (human) xenograft tissue stained for DPF2/REQ using ab227095 at 1/250 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227095 has not yet been referenced specifically in any publications.