Overview

  • Product name

  • Description

    Rabbit polyclonal to DPF2/REQ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Chimpanzee
  • Immunogen

    Recombinant fragment within Human DPF2/REQ (internal sequence). The exact sequence is proprietary.
    Database link: Q92785

  • Positive control

    • IHC-P: H661 xenograft tissue; Mouse brain tissue. WB: DPF2/REQ transfected HEK-293T cell lysate.
  • General notes

     This product was previously labelled as DPF2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.

Target

  • Function

    May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the requiem/DPF family.
    Contains 1 C2H2-type zinc finger.
    Contains 2 PHD-type zinc fingers.
  • Cellular localization

    Nucleus. Cytoplasm. 30% nuclear. 70% cytoplasmic.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis response zinc finger protein antibody
    • BAF45D antibody
    • BRG1-associated factor 45D antibody
    • D4 antibody
    • D4 zinc and double PHD fingers family 2 antibody
    • Double PHD fingers 2 antibody
    • double PHD fingers family 2 antibody
    • DPF 2 antibody
    • DPF2 antibody
    • MGC10180 antibody
    • Protein requiem antibody
    • REQ antibody
    • REQU_HUMAN antibody
    • Requiem antibody
    • Requiem apoptosis response zinc finger antibody
    • UBI D4 antibody
    • ubi-d4 antibody
    • UBID 4 antibody
    • UBID4 antibody
    • zinc and double PHD fingers family 2 antibody
    • Zinc finger protein ubi d4 antibody
    • Zinc finger protein ubi-d4 antibody
    see all

Images

  • Paraffin-embedded mouse brain tissue stained for DPF2/REQ using ab227095 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-DPF2/REQ antibody (ab227095) at 1/500 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : DPF2/REQ transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Predicted band size: 44 kDa



    12% SDS-PAGE gel.

  • Paraffin-embedded H661 xenograft tissue stained for DPF2/REQ using ab227095 at 1/250 dilution in immunohistochemical analysis.

References

ab227095 has not yet been referenced specifically in any publications.

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