Overview

  • Product name

  • Description

    Rabbit polyclonal to DPF3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human DPF3 aa 108-157.
    Sequence:

    KDGFTSESTTLEALLRGEGVEKKVDAREEESIQEIQRVLENDENVEEGNE


    Database link: NP_036206

  • Positive control

    • MCF7 cell lysate
  • General notes

    Previously labelled as CERD4.

Properties

Applications

Our Abpromise guarantee covers the use of ab85360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development.
  • Sequence similarities

    Belongs to the requiem/DPF family.
    Contains 1 C2H2-type zinc finger.
    Contains 2 PHD-type zinc fingers.
  • Domain

    The PHD-type zinc fingers mediate the binding to acetylated histones.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2810403B03Rik antibody
    • BAF45C antibody
    • BRG1-associated factor 45C antibody
    • cer-d4 homolog antibody
    • CERD4 antibody
    • D4 zinc and double PHD fingers family member 3 antibody
    • D4, zinc and double PHD fingers, family 3 antibody
    • DPF 3 antibody
    • Dpf3 antibody
    • DPF3_HUMAN antibody
    • FLJ 14079 antibody
    • FLJ14079 antibody
    • Mouse homolog of CER D4 antibody
    • Zinc finger protein cer-d4 antibody
    • Zinc finger protein DPF3 antibody
    see all

Images

  • Anti-DPF3 antibody (ab85360) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

  • Western Blot using ab85360 at 1.0ug/ml. Sample type: Human HT1080 cell Lysate
  • Western Blot using ab85360 at 1.0ug/ml. Sample type: Human NCI-H226 cell Lysate

References

This product has been referenced in:

  • Theodorou M  et al. Identification of a STAT5 Target Gene, Dpf3, Provides Novel Insights in Chronic Lymphocytic Leukemia. PLoS One 8:e76155 (2013). Read more (PubMed: 24155890) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Q&A

Answer

DISCOUNT CODE: xxxxx
Expiration date: 2nd of March 2012
Value: €370

I am very pleased to hear you would like to accept our offer and test ab85360 in western blotting with your mouse samples. This code will give you €370 off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse/WB and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site:

www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

www.abcam.com/abtrial

Read More

Answer

Thank you very much for your reply.

I am pleased to hear you would like to participate in the Abtrial program. In order to raise a discount code for you, could you just confirm which antibody it is you would like to purchase to test in the scheme? Ab85360 or ab127703?

I look forward to receiving your reply.

Read More

Question
Answer

Thank you for contacting us and your interest in our products.

As we discussed over the phone, I have looked into the two anti-CERD4 antibodies we currently have in the catalogue and the likelihood that they would cross react with your mouse samples. Following sequence alignment of the immunogens used to raise the ab85360 and ab127703 against the mouse protein I found that they shared 100 and 99% homology respectively with the mouse protein. Both would therefore be likely to cross react. However, as we have not tested this ourselves as yet we would not be able to guarantee it.

If you would like to try either of the antibodies with your mouse samples in Western blotting you would be eligible for our Abtrial testing discount scheme.

This offer means that if you purchase ab85360 or ab127703 as normal, test the antibody with your mouse samples and let us know of the results through an Abreview (no matter whether positive or negative you would then be eligible to claim the value of ab85360 or ab127703 off any product in our catalogue. More information on this offer can be found here:

www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab85360 or ab127703.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up