Overview

  • Product name
  • Description
    Rabbit polyclonal to DPF3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human DPF3 aa 108-157.
    Sequence:

    KDGFTSESTTLEALLRGEGVEKKVDAREEESIQEIQRVLENDENVEEGNE


    Database link: NP_036206

  • Positive control
    • MCF7 cell lysate
  • General notes

    Previously labelled as CERD4.

Properties

Applications

Our Abpromise guarantee covers the use of ab85360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development.
  • Sequence similarities
    Belongs to the requiem/DPF family.
    Contains 1 C2H2-type zinc finger.
    Contains 2 PHD-type zinc fingers.
  • Domain
    The PHD-type zinc fingers mediate the binding to acetylated histones.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2810403B03Rik antibody
    • BAF45C antibody
    • BRG1-associated factor 45C antibody
    • cer-d4 homolog antibody
    • CERD4 antibody
    • D4 zinc and double PHD fingers family member 3 antibody
    • D4, zinc and double PHD fingers, family 3 antibody
    • DPF 3 antibody
    • Dpf3 antibody
    • DPF3_HUMAN antibody
    • FLJ 14079 antibody
    • FLJ14079 antibody
    • Mouse homolog of CER D4 antibody
    • Zinc finger protein cer-d4 antibody
    • Zinc finger protein DPF3 antibody
    see all

Images

  • Anti-DPF3 antibody (ab85360) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

  • Western Blot using ab85360 at 1.0ug/ml. Sample type: Human HT1080 cell Lysate
  • Western Blot using ab85360 at 1.0ug/ml. Sample type: Human NCI-H226 cell Lysate

References

This product has been referenced in:
  • Theodorou M  et al. Identification of a STAT5 Target Gene, Dpf3, Provides Novel Insights in Chronic Lymphocytic Leukemia. PLoS One 8:e76155 (2013). Read more (PubMed: 24155890) »
See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mr. Huanhuan Cui

Verified customer

Submitted Jan 10 2013

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