Key features and details
- Rabbit polyclonal to DPH4
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DPH4 antibody
See all DPH4 primary antibodies
DescriptionRabbit polyclonal to DPH4
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- IHC-P: Human duodenum tissue. WB: DPH4 overexpression HEK-293T cell lysate. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionStimulates the ATPase activity of several Hsp70-type chaperones.
Sequence similaritiesBelongs to the DPH4 family.
Contains 1 DPH-type zinc finger.
Contains 1 J domain.
- Information by UniProt
- 1700030A21Rik antibody
- 2610027M02Rik antibody
- AV066965 antibody
PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling DPH4 using DPH4 at 4 µg/ml (green) in ICC/IF.
Paraffin-embedded human duodenum tissue stained for DPH4 using ab246925 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-DPH4 antibody (ab246925) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : DPH4 overexpression HEK-293T cell lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246925 has not yet been referenced specifically in any publications.