Key features and details
- Mouse polyclonal to DPH5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DPH5 antibody
DescriptionMouse polyclonal to DPH5
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length DPH5 protein (Human) (NP_001070862.1)
- DPH5 transfected 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab69347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).|
RelevanceDPH5 is a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. DPH5 is required for the trimethylation step of diphthamide biosynthesis. There are five named isoforms.
- AD 018 antibody
- AD018 antibody
- CGI 30 antibody
All lanes : Anti-DPH5 antibody (ab69347) at 1/500 dilution
Lane 1 : DPH5 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
ab69347 has not yet been referenced specifically in any publications.