Overview

  • Product name

    Anti-DPL antibody
  • Description

    Rabbit polyclonal to DPL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human DPL aa 112-120.
    Sequence:

    TQAANQGEF

  • Positive control

    • Mouse spleen 100k x g supernatant for Western blot.
  • General notes

     This product was previously labelled as Doppel

     

Properties

Applications

Our Abpromise guarantee covers the use of ab23701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P Use a concentration of 1 µg/ml.

Target

Images

  • All lanes : Anti-DPL antibody (ab23701) at 2 µg/ml

    Lane 1 : 27ug mouse spleen 100k x g supernatant.
    Lane 2 : 54ug mouse spleen 100k x g supernatant.

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 25 kDa (possible glycosylated form)

References

ab23701 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (CNS)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
CNS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 24 2017

Application
Western blot
Sample
Human Tissue lysate - whole (CNS)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
CNS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 18 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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