Key features and details
- Rabbit polyclonal to DPM1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DPM1 antibody
See all DPM1 primary antibodies
DescriptionRabbit polyclonal to DPM1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Pig
Recombinant full length protein corresponding to Human DPM1 aa 2-260.
ASLEVSRSPRRSRRELEVRSPRQNKYSVLLPTYNERENLPLIVWLLVKSF SESGINYEIIIIDDGSPDGTRDVAEQLEKIYGSDRILLRPREKKLGLGTA YIHGMKHATGNYIIIMDADLSHHPKFIPEFIRKQKEGNFDIVSGTRYKGN GGVYGWDLKRKIISRGANFLTQILLRPGASDLTGSFRLYRKEVLEKLIEK CVSKGYVFQMEMIVRARQLNYTIGEVPISFVDRVYGESKLGGNEIVSFLK GLLTLFATT
Database link: O60762
- WB: Mouse lung lysate. IHC-P: Human kidney and pancreas tissue. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 30 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionTransfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.
PathwayProtein modification; protein glycosylation.
Involvement in diseaseDefects in DPM1 are the cause of congenital disorder of glycosylation type 1E (CDG1E) [MIM:608799]. CDGs are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation. They are characterized by under-glycosylated serum glycoproteins. CDG1E is an autosomal recessive disorder, characterized by severe developmental delay, hypotnia, seizures, and dysmorphic features.
Sequence similaritiesBelongs to the glycosyltransferase 2 family.
Cellular localizationEndoplasmic reticulum.
- Information by UniProt
- CDGIE antibody
- dolichol monophosphate mannose synthase antibody
- Dolichol phosphate mannose synthase antibody
Anti-DPM1 antibody (ab236604) at 1/500 dilution + Mouse lung lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30 kDa
Paraffin-embedded human kidney tissue stained for DPM1 using ab236604 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreas tissue stained for DPM1 using ab236604 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for DPM1 (green) using ab236604 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236604 has not yet been referenced specifically in any publications.