Overview

  • Product name
    Anti-DPM3 antibody
    See all DPM3 primary antibodies
  • Description
    Goat polyclonal to DPM3
  • Host species
    Goat
  • Specificity
    ab175112 is expected to recognize both reported isoforms (NP_061846.2 and NP_714963.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig
  • Immunogen

    Synthetic peptide within Human DPM3 aa 65-77 (internal sequence). The exact sequence is proprietary. NP_714963.1.
    Sequence:

    HDCEDAARELQSQ


    Database link: Q9P2X0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • K562 lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab175112 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 10 kDa.

Target

  • Relevance
    Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. DPM3 is a subunit of dolichyl-phosphate mannosyltransferase and acts as a stabilizer subunit of the dolichyl-phosphate mannosyltransferase complex.
  • Cellular localization
    Integral membrane protein. Endoplasmic reticulum.
  • Database links
    see all
  • Alternative names
    • CDG1O antibody
    • Dolichol Phosphate Mannose Synthase subunit 3 antibody
    • Dolichol phosphate mannosyltransferase subunit 3 antibody
    • Dolichyl Phosphate beta D Mannosyltransferase subunit 3 antibody
    • Dolichyl phosphate mannosyltransferase polypeptide 3 antibody
    • DPM synthase complex subunit 3 antibody
    • Mannose P Dolichol Synthase subunit 3 antibody
    • MPD synthase subunit 3 antibody
    • Prostin 1 antibody
    see all

Images

  • Western blot - Anti-DPM3 antibody (ab175112)
    Western blot - Anti-DPM3 antibody (ab175112)
    Anti-DPM3 antibody (ab175112) at 3 µg/ml + K562 lysate at 35 µg

    Predicted band size: 10 kDa

References

ab175112 has not yet been referenced specifically in any publications.

Publishing research using ab175112? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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