Anti-DPM3 antibody (ab175112)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-DPM3 antibody
See all DPM3 primary antibodies -
DescriptionGoat polyclonal to DPM3
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Host speciesGoat
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Specificityab175112 is expected to recognize both reported isoforms (NP_061846.2 and NP_714963.1).
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig
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Immunogen
Synthetic peptide within Human DPM3 aa 65-77 (internal sequence). The exact sequence is proprietary. NP_714963.1.
Sequence:HDCEDAARELQSQ
Database link: Q9P2X0 -
Positive control
- K562 lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab175112 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 10 kDa. |
Target
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RelevanceDolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. DPM3 is a subunit of dolichyl-phosphate mannosyltransferase and acts as a stabilizer subunit of the dolichyl-phosphate mannosyltransferase complex.
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Cellular localizationIntegral membrane protein. Endoplasmic reticulum.
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Database links
- Entrez Gene: 490430 Dog
- Entrez Gene: 54344 Human
- Entrez Gene: 68563 Mouse
- Entrez Gene: 502017 Rat
- Omim: 605951 Human
- SwissProt: Q9P2X0 Human
- SwissProt: Q9D1Q4 Mouse
- Unigene: 110477 Human
see all -
Alternative names
- CDG1O antibody
- Dolichol Phosphate Mannose Synthase subunit 3 antibody
- Dolichol phosphate mannosyltransferase subunit 3 antibody
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Images
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Western blot - Anti-DPM3 antibody (ab175112)Anti-DPM3 antibody (ab175112) at 3 µg/ml + K562 lysate at 35 µg
Predicted band size: 10 kDa
Protocols
Datasheets and documents
References
ab175112 has not yet been referenced specifically in any publications.
