Anti-DPP3 antibody (ab217127)
Key features and details
- Rabbit polyclonal to DPP3
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DPP3 antibody
See all DPP3 primary antibodies -
Description
Rabbit polyclonal to DPP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human DPP3 aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9NY33 -
Positive control
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7.0 to 8.0 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab217127 was affinity purified using an epitope specific to DPP3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 83 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 83 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Cleaves Arg-Arg-beta-naphthylamide. -
Sequence similarities
Belongs to the peptidase M49 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10072 Human
- Entrez Gene: 75221 Mouse
- Entrez Gene: 114591 Rat
- Omim: 606818 Human
- SwissProt: Q9NY33 Human
- SwissProt: Q99KK7 Mouse
- SwissProt: O55096 Rat
- Unigene: 502914 Human
see all -
Alternative names
- Dipeptidyl aminopeptidase III antibody
- Dipeptidyl arylamidase III antibody
- Dipeptidyl peptidase 3 antibody
see all
Images
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All lanes : Anti-DPP3 antibody (ab217127) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 83 kDa
Exposure time: 30 secondsLysis buffer: NETN
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Detection of DPP3 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217127 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab217127 was used at 1µg/ml. Lane 2 Control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab217127 has not yet been referenced specifically in any publications.