Overview

  • Product name

  • Description

    Rabbit polyclonal to DPP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 138 and 435 of DPP3 (NP_005691).

  • Positive control

    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; IHC: AGS xenograft. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

Images

  • Anti-DPP3 antibody (ab97437) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 83 kDa

  • Top: immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97437 at 1/200 dilution. Bottom: image merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded AGS xenograft, using ab97437 at 1/100 dilution.

References

This product has been referenced in:

  • Hast BE  et al. Cancer-derived mutations in KEAP1 impair NRF2 degradation but not ubiquitination. Cancer Res 74:808-17 (2014). Human . Read more (PubMed: 24322982) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse liver)
Permeabilization
No
Specification
mouse liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Sandra Sobocanec

Verified customer

Submitted Oct 06 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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