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    dpp3-antibody-epr9020b-ab133671.pdf

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Cell Biology Proteolysis / Ubiquitin Proteolytic enzymes Serine protease DPPs
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RecombinantRabMAb

Recombinant Anti-DPP3 antibody [EPR9020(B)] (ab133671)

  • Datasheet
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Western blot - Anti-DPP3 antibody [EPR9020(B)] (ab133671)
  • Flow Cytometry - Anti-DPP3 antibody [EPR9020(B)] (ab133671)
  • Anti-DPP3 antibody [EPR9020(B)] (ab133671)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9020(B)] to DPP3
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant human DPP3 protein (ab79652)

View more associated products

Overview

  • Product name

    Anti-DPP3 antibody [EPR9020(B)]
    See all DPP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR9020(B)] to DPP3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to a region within Human DPP3 (Q9NY33).

  • Positive control

    • Human fetal liver lysate, HeLa cell lysate, 293T cell lysate, U87 MG cells and cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9020(B)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Serine protease
    • DPPs

Associated products

  • Alternative Versions

    • Anti-DPP3 antibody [EPR9020(B)] - BSA and Azide free (ab248606)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human DPP3 protein (ab79652)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/10000 - 1/50000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Cleaves Arg-Arg-beta-naphthylamide.
    • Sequence similarities

      Belongs to the peptidase M49 family.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession Q9NY33 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10072 Human
      • Omim: 606818 Human
      • SwissProt: Q9NY33 Human
      • Unigene: 502914 Human
      • Alternative names

        • Dipeptidyl aminopeptidase III antibody
        • Dipeptidyl arylamidase III antibody
        • Dipeptidyl peptidase 3 antibody
        • Dipeptidyl peptidase III antibody
        • DPP III antibody
        • dpp3 antibody
        • DPP3_HUMAN antibody
        • DPPIII antibody
        • EC 3.4.14.4 antibody
        see all

      Images

      • Western blot - Anti-DPP3 antibody [EPR9020(B)] (ab133671)
        Western blot - Anti-DPP3 antibody [EPR9020(B)] (ab133671)
        All lanes : Anti-DPP3 antibody [EPR9020(B)] (ab133671) at 1/10000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : 293T cell lysate
        Lane 4 : U87 MG cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

        Predicted band size: 83 kDa

      • Flow Cytometry - Anti-DPP3 antibody [EPR9020(B)] (ab133671)
        Flow Cytometry - Anti-DPP3 antibody [EPR9020(B)] (ab133671)
        Flow cytometric analysis of permeabilized U87 MG cells labelling DPP3 with ab133671 at 1/100 (red) or with a negative control rabbit IgG (green).
      • Anti-DPP3 antibody [EPR9020(B)] (ab133671)
        Anti-DPP3 antibody [EPR9020(B)] (ab133671)

      Protocols

      • Flow cytometry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab133671? Please let us know so that we can cite the reference in this datasheet.

      ab133671 has been referenced in 1 publication.

      • Lu K  et al. NRF2 Induction Supporting Breast Cancer Cell Survival Is Enabled by Oxidative Stress-Induced DPP3-KEAP1 Interaction. Cancer Res 77:2881-2892 (2017). PubMed: 28416489

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

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