RecombinantRabMAb

Recombinant Anti-DPP3 antibody [EPR9020(B)] (ab133671)

Submit a review Submit a question References (1)
Western blot - Anti-DPP3 antibody [EPR9020(B)] (ab133671)
  • Flow Cytometry - Anti-DPP3 antibody [EPR9020(B)] (ab133671)
  • Anti-DPP3 antibody [EPR9020(B)] (ab133671)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9020(B)] to DPP3
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-DPP3 antibody [EPR9020(B)]
    See all DPP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR9020(B)] to DPP3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region within Human DPP3 (Q9NY33).

  • Positive control

    • Human fetal liver lysate, HeLa cell lysate, 293T cell lysate, U87 MG cells and cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9020(B)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Cleaves Arg-Arg-beta-naphthylamide.

    • Sequence similarities

      Belongs to the peptidase M49 family.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • Dipeptidyl aminopeptidase III antibody
        • Dipeptidyl arylamidase III antibody
        • Dipeptidyl peptidase 3 antibody
        • Dipeptidyl peptidase III antibody
        • DPP III antibody
        • dpp3 antibody
        • DPP3_HUMAN antibody
        • DPPIII antibody
        • EC 3.4.14.4 antibody
        see all

      Images

      • Western blot - Anti-DPP3 antibody [EPR9020(B)] (ab133671)
        Western blot - Anti-DPP3 antibody [EPR9020(B)] (ab133671)
        All lanes : Anti-DPP3 antibody [EPR9020(B)] (ab133671) at 1/10000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : 293T cell lysate
        Lane 4 : U87 MG cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

        Predicted band size: 83 kDa

      • Flow Cytometry - Anti-DPP3 antibody [EPR9020(B)] (ab133671)
        Flow Cytometry - Anti-DPP3 antibody [EPR9020(B)] (ab133671)
        Flow cytometric analysis of permeabilized U87 MG cells labelling DPP3 with ab133671 at 1/100 (red) or with a negative control rabbit IgG (green).
      • Anti-DPP3 antibody [EPR9020(B)] (ab133671)
        Anti-DPP3 antibody [EPR9020(B)] (ab133671)

      References (1)

      Publishing research using ab133671? Please let us know so that we can cite the reference in this datasheet.

      ab133671 has been referenced in 1 publication.

      • Lu K  et al. NRF2 Induction Supporting Breast Cancer Cell Survival Is Enabled by Oxidative Stress-Induced DPP3-KEAP1 Interaction. Cancer Res 77:2881-2892 (2017). PubMed: 28416489

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab133671.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com