Overview

  • Product name

    Anti-DPP3 antibody [EPR9020(B)]
    See all DPP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR9020(B)] to DPP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human DPP3 (Q9NY33).

  • Positive control

    • Human fetal liver lysate, HeLa cell lysate, 293T cell lysate, U87 MG cells and cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9020(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Cleaves Arg-Arg-beta-naphthylamide.
    • Sequence similarities

      Belongs to the peptidase M49 family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Dipeptidyl aminopeptidase III antibody
      • Dipeptidyl arylamidase III antibody
      • Dipeptidyl peptidase 3 antibody
      • Dipeptidyl peptidase III antibody
      • DPP III antibody
      • dpp3 antibody
      • DPP3_HUMAN antibody
      • DPPIII antibody
      • EC 3.4.14.4 antibody
      see all

    Images

    • All lanes : Anti-DPP3 antibody [EPR9020(B)] (ab133671) at 1/10000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : U87 MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 83 kDa

    • Flow cytometric analysis of permeabilized U87 MG cells labelling DPP3 with ab133671 at 1/100 (red) or with a negative control rabbit IgG (green).

    References

    This product has been referenced in:

    • Lu K  et al. NRF2 Induction Supporting Breast Cancer Cell Survival Is Enabled by Oxidative Stress-Induced DPP3-KEAP1 Interaction. Cancer Res 77:2881-2892 (2017). Read more (PubMed: 28416489) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133671.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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