Recombinant Anti-DPP3 antibody [EPR9020(B)] (ab133671)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-DPP3 antibody [EPR9020(B)]
See all DPP3 primary antibodies -
Description
Rabbit monoclonal [EPR9020(B)] to DPP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within Human DPP3 (Q9NY33).
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Positive control
- Human fetal liver lysate, HeLa cell lysate, 293T cell lysate, U87 MG cells and cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9020(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). | |
| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Cleaves Arg-Arg-beta-naphthylamide. -
Sequence similarities
Belongs to the peptidase M49 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10072 Human
- Omim: 606818 Human
- SwissProt: Q9NY33 Human
- Unigene: 502914 Human
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Alternative names
- Dipeptidyl aminopeptidase III antibody
- Dipeptidyl arylamidase III antibody
- Dipeptidyl peptidase 3 antibody
see all
Images
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![Western blot - Anti-DPP3 antibody [EPR9020(B)] (ab133671)](https://www.abcam.com/ps/datasheet/images/133/ab133671/DPP3-Primary-antibodies-ab133671-1.jpg)
Western blot - Anti-DPP3 antibody [EPR9020(B)] (ab133671)All lanes : Anti-DPP3 antibody [EPR9020(B)] (ab133671) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : U87 MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 83 kDa -
![Flow Cytometry - Anti-DPP3 antibody [EPR9020(B)] (ab133671)](https://www.abcam.com/ps/datasheet/images/133/ab133671/DPP3-Primary-antibodies-ab133671-2.jpg)
Flow Cytometry - Anti-DPP3 antibody [EPR9020(B)] (ab133671)Flow cytometric analysis of permeabilized U87 MG cells labelling DPP3 with ab133671 at 1/100 (red) or with a negative control rabbit IgG (green).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Lu K et al. NRF2 Induction Supporting Breast Cancer Cell Survival Is Enabled by Oxidative Stress-Induced DPP3-KEAP1 Interaction. Cancer Res 77:2881-2892 (2017). Read more (PubMed: 28416489) »
