Recombinant Anti-DPP3 antibody [EPR9020(B)] (ab133671)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9020(B)] to DPP3
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-DPP3 antibody [EPR9020(B)]
See all DPP3 primary antibodies -
Description
Rabbit monoclonal [EPR9020(B)] to DPP3 -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide corresponding to a region within Human DPP3 (Q9NY33).
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Positive control
- Human fetal liver lysate, HeLa cell lysate, 293T cell lysate, U87 MG cells and cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9020(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
|
|
Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Cleaves Arg-Arg-beta-naphthylamide. -
Sequence similarities
Belongs to the peptidase M49 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10072 Human
- Omim: 606818 Human
- SwissProt: Q9NY33 Human
- Unigene: 502914 Human
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Alternative names
- Dipeptidyl aminopeptidase III antibody
- Dipeptidyl arylamidase III antibody
- Dipeptidyl peptidase 3 antibody
see all
Images
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All lanes : Anti-DPP3 antibody [EPR9020(B)] (ab133671) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : U87 MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 83 kDa -
Flow cytometric analysis of permeabilized U87 MG cells labelling DPP3 with ab133671 at 1/100 (red) or with a negative control rabbit IgG (green).
Datasheets and documents
References (1)
ab133671 has been referenced in 1 publication.
- Lu K et al. NRF2 Induction Supporting Breast Cancer Cell Survival Is Enabled by Oxidative Stress-Induced DPP3-KEAP1 Interaction. Cancer Res 77:2881-2892 (2017). PubMed: 28416489