Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9021(B)] to DPP3
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-DPP3 antibody [EPR9021(B)]
See all DPP3 primary antibodies
DescriptionRabbit monoclonal [EPR9021(B)] to DPP3
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human DPP3 aa 700-800 (C terminal). The exact sequence is proprietary.
- HeLa cells. Human fetal liver, HeLa, 293T, U87-MG cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C.
Dissociation constant (KD)KD = 6.60 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 83 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Sequence similaritiesBelongs to the peptidase M49 family.
- Information by UniProt
- Dipeptidyl aminopeptidase III antibody
- Dipeptidyl arylamidase III antibody
- Dipeptidyl peptidase 3 antibody
All lanes : Anti-DPP3 antibody [EPR9021(B)] (ab133735) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 83 kDa
Flow cytometric analysis of DPP3 expression in permeabilized HeLa cells using ab133735 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133735 has been referenced in 1 publication.
- Lu K et al. NRF2 Induction Supporting Breast Cancer Cell Survival Is Enabled by Oxidative Stress-Induced DPP3-KEAP1 Interaction. Cancer Res 77:2881-2892 (2017). PubMed: 28416489