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    dpp4-antibody-ab28340.pdf

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Immunology Adaptive Immunity B Cells CD
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Anti-DPP4 antibody (ab28340)

  • Datasheet
  • SDS
Reviews (8)Q&A (3)References (37)

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Western blot - Anti-DPP4 antibody (ab28340)

    Key features and details

    • Rabbit polyclonal to DPP4
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-DPP4 antibody [EPR20819] (ab215711)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-DPP4 antibody
      See all DPP4 primary antibodies
    • Description

      Rabbit polyclonal to DPP4
    • Host species

      Rabbit
    • Specificity

      Does not recognize other DPP family members.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Recombinant fragment
    • Immunogen

      Synthetic peptide corresponding to Human DPP4. Based on the spacer region of Human DPP4, mid-molecule, before the cysteine-rich region.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituent: 50% Glycerol
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      The antibody has been peptide-affinity purified.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage
      • Immunology
      • Cell Type Markers
      • CD
      • Adhesion
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Serine protease
      • DPPs
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • HIV-related
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human DPP4 protein (His tag) (ab155641)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab28340 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (3)
    1/1000 - 1/5000. Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Bands run high because of post-translational modifications and reduction. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.
    Notes
    WB
    1/1000 - 1/5000. Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Bands run high because of post-translational modifications and reduction. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.

    Target

    • Function

      Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
    • Tissue specificity

      Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
    • Sequence similarities

      Belongs to the peptidase S9B family. DPPIV subfamily.
    • Domain

      The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.
    • Post-translational
      modifications

      The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
      N- and O-Glycosylated.
      Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
    • Cellular localization

      Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.
    • Target information above from: UniProt accession P27487 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • CD26 antigen antibody
      • ADA-binding protein antibody
      • ADABP antibody
      • ADCP 2 antibody
      • ADCP-2 antibody
      • ADCP2 antibody
      • Adenosine deaminase complexing protein 2 antibody
      • CD 26 antibody
      • CD26 antibody
      • CD26 antigen 3 antibody
      • Dipeptidyl peptidase 4 antibody
      • Dipeptidyl peptidase 4 soluble form antibody
      • Dipeptidyl peptidase IV antibody
      • Dipeptidyl peptidase IV membrane form antibody
      • Dipeptidyl peptidase IV soluble form antibody
      • Dipeptidyl peptidase, intestinal antibody
      • Dipeptidylpeptidase 4 antibody
      • Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2) antibody
      • Dipeptidylpeptidase IV antibody
      • DPP 4 antibody
      • DPP IV antibody
      • DPP IV estoenzyme antibody
      • DPP4 antibody
      • DPP4_HUMAN antibody
      • DPPIV antibody
      • Intestinal dipeptidyl peptidase antibody
      • T cell activation antigen CD26 antibody
      • T-cell activation antigen CD26 antibody
      • TP 103 antibody
      • TP103 antibody
      see all

    Images

    • Western blot - Anti-DPP4 antibody (ab28340)
      Western blot - Anti-DPP4 antibody (ab28340)
      All lanes : Anti-DPP4 antibody (ab28340) at 1/1000 dilution

      Lane 1 : DPP-4 at 0.08 µg
      Lane 2 : DPP-4 at 0.04 µg
      Lane 3 : DPP-4 at 0.02 µg
      Lane 4 : DPP-4 at 0.01 µg

      Predicted band size: 88 kDa
      Observed band size: 115 kDa why is the actual band size different from the predicted?



      Postranslational modifications and reduction lead to an apparent mass of 110-120 kDa on SDS-PAGE gels.

    Protocols

    • Recommended WB protocol

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (37)

    Publishing research using ab28340? Please let us know so that we can cite the reference in this datasheet.

    ab28340 has been referenced in 37 publications.

    • Zhu X  et al. HDAC1/2 Control Proliferation and Survival in Adult Epidermis and Pre?Basal Cell Carcinoma through p16 and p53. J Invest Dermatol 142:77-87.e10 (2022). PubMed: 34284046
    • Yu H  et al. A poor prognosis in human hepatocellular carcinoma is associated with low expression of DPP4. Braz J Med Biol Res 53:e9114 (2020). PubMed: 32294701
    • Li S  et al. Deficiency in Dipeptidyl Peptidase-4 Promotes Chemoresistance through the CXCL12/CXCR4/mTOR/TGFß Signaling Pathway in Breast Cancer Cells. Int J Mol Sci 21:N/A (2020). PubMed: 31991851
    • Li YP  et al. DPP-4 inhibition resembles exercise in preventing type 2 diabetes development by inhibiting hepatic protein kinase Ce expression in a mouse model of hyperinsulinemia. J Int Med Res 48:300060520934635 (2020). PubMed: 32588693
    • Falasca L  et al. Postmortem Findings in Italian Patients With COVID-19: A Descriptive Full Autopsy Study of Cases With and Without Comorbidities. J Infect Dis 222:1807-1815 (2020). PubMed: 32914853
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 11 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DPP4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    Yes - Triton
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Radhika Atit

    Verified customer

    Submitted Jul 19 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DPP4 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted Sep 14 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DPP4 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted Sep 14 2018

    Western blot abreview for Anti-CD26 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Sample
    Mouse Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 02 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DPP4 antibody

    Average
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
    Permeabilization
    No
    Specification
    liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 05 2014

    Western blot abreview for Anti-CD26 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Tissue lysate - whole (bovine epididymis)
    Loading amount
    40 µg
    Specification
    bovine epididymis
    Treatment
    homogenized in 0.02 M Hepes buffer, pH 7.0, containing 1% octylglucoside and a complete protease inhibitor cocktail tablet
    Gel Running Conditions
    Reduced Denaturing (7,5% gel)
    Blocking step
    0.05 M Tris buffer containing 0.15 M NaCl, 0.1% Tween 20, 5% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Dr. Veronique Dubois

    Verified customer

    Submitted Aug 05 2009

    Western blot abreview for Anti-CD26 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Purified protein (human seminal plasma)
    Loading amount
    0.32 µg
    Specification
    human seminal plasma
    Gel Running Conditions
    Reduced Denaturing (7,5% gel)
    Blocking step
    0.05 M Tris buffer containing 0.15 M NaCl, 5 mM NaN3 and 2% Tween 20 as blocking agent for 1 hour(s) and 0 minute(s) · Temperature: 25°C
    Read More

    Dr. Veronique Dubois

    Verified customer

    Submitted Jun 29 2009

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD26 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Epididymis)
    Specification
    Epididymis
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer pH 9.0
    Permeabilization
    No
    Blocking step
    3% H2O2 in TBS as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Dr. Veronique Dubois

    Verified customer

    Submitted Jun 17 2009

    Question

    Muchas gracias. Una de mis dudas es cual CD26 sería adecuado porque veo

    que uno detecta 100 KDa, otro dos fragmentos menores.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 05 2012

    Answer

    En principio ab28340 detecta una banda a un peso algo superior al esperado debido a modificaciones post translacionales, el otro, ab114033 muestra una banda a un peso molecular menor que también aparece en la calle que contiene el control a mucha menor concentración.

    No tenemos evidencia del origen de esa banda, es posible que se deba al procesado de CD26, pero no hay datos que lo confirmen.

    Siento no poder ser de más ayuda en esta ocasión. Si necesitas algo mas, no dudes en consultarnos de nuevo.

    Read More

    Abcam Scientific Support

    Answered on Dec 05 2012

    Question

     Dear Sir/Madam,  We have troubles with the detection of the CD26 protein (antibody of COMPETITOR NAME) in our proximal tubule cell line, although we know that it should be present in these cells. On the Abcam website I found your antibody product ab28340, 100ug for 370euro. Because of the prize we would like to test a small amount of this antibody first, before we order this expensive antibody.  Is it possible that we receive some ul’s of your antibody so we can perform a Westernblot on a few samples to test the specificity and detection properties of your antibody?  With kind regards,    

    Read More

    Abcam community

    Verified customer

    Asked on Nov 11 2011

    Answer

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. This antibody is tested and guaranteed for WB and IHC-P in Mouse, Rat, and Human samples at this moment. We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact us again with any other questions.  

    Read More

    Abcam Scientific Support

    Answered on Nov 11 2011

    1-10 of 11 Abreviews or Q&A

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