Recombinant Anti-DPP4 antibody [EPR18215] (ab187048)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (ab187048)](https://www.abcam.com/ps/products/187/ab187048/Images/ab187048-285375-anti-cd26-antibody-epr18215-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (ab187048)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18215] to DPP4
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-DPP4 antibody [EPR18215]
See all DPP4 primary antibodies -
Description
Rabbit monoclonal [EPR18215] to DPP4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseRatIHC-P MouseRatWB MouseRat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat lung, thymus, liver and kidney tissue lysate; mouse lung and thymus tissue lysate. IHC-P: Mouse liver tissue; rat liver tissue. IHC-Fr: Mouse liver tissue; rat liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18215 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ELISA |
Human
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| IHC-Fr |
Mouse
Rat
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| IHC-P |
Mouse
Rat
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| WB |
Mouse
Rat
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
|
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| IHC-P | (2) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IHC-Fr |
1/200 - 1/1000.
Rat: use at 1/200 Mouse: use at 1/1000 |
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa). |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IHC-Fr
1/200 - 1/1000. Rat: use at 1/200 Mouse: use at 1/1000 |
Target
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Function
Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. -
Tissue specificity
Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon. -
Sequence similarities
Belongs to the peptidase S9B family. DPPIV subfamily. -
Domain
The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity. -
Post-translational
modificationsThe soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
N- and O-Glycosylated.
Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation. -
Cellular localization
Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid. - Information by UniProt
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Database links
- Entrez Gene: 13482 Mouse
- Entrez Gene: 25253 Rat
- SwissProt: P28843 Mouse
- SwissProt: P14740 Rat
- Unigene: 1151 Mouse
- Unigene: 91364 Rat
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Alternative names
- CD26 antigen antibody
- ADA-binding protein antibody
- ADABP antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (ab187048)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD26 with ab187048 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on bile canaliculi of mouse liver is observed (PMID: 23361237). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Western blot - Anti-DPP4 antibody [EPR18215] (ab187048)](https://www.abcam.com/ps/products/187/ab187048/Images/ab187048-285374-anti-cd26-antibody-epr18215-western-blot.jpg)
Western blot - Anti-DPP4 antibody [EPR18215] (ab187048)All lanes : Anti-DPP4 antibody [EPR18215] (ab187048) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Rat lung lysate
Lane 3 : Mouse thymus lysate
Lane 4 : Rat thymus lysate
Lane 5 : Rat liver lysate
Lane 6 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))
Predicted band size: 88 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST
Exposure times.
Lanes 1, 3, 4: 30 seconds
Lane 2: 20 seconds
Lane 5: 10 seconds
Lane 6: 15 seconds
The molecular weight observed is consistent with what has been described in the literature (PMID: 25589660).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (ab187048)](https://www.abcam.com/ps/products/187/ab187048/Images/ab187048-285382-anti-cd26-antibody-epr18215-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (ab187048)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling CD26 with ab187048 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on bile canaliculi of rat liver is observed (PMID: 23361237). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (ab187048)](https://www.abcam.com/ps/products/187/ab187048/Images/ab187048-285383-anti-cd26-antibody-epr18215-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (ab187048)Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized mouse liver tissue labeling CD26 with ab187048 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Strong positive staining on the bile canaliculi and moderate positive staining in sinusoid endothelial cells is observed (PMID: 23361237). The nuclear counter stain is DAPI (blue)
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.Antigen retrieval: Heated Tris-EDTA (pH9.0) (ab94681).
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![Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (ab187048)](https://www.abcam.com/ps/products/187/ab187048/Images/ab187048-285386-anti-cd26-antibody-epr18215-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (ab187048)Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized rat liver tissue labeling CD26 with ab187048 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Strong positive staining on the bile canaliculi and moderate positive staining in sinusoid endothelial cells is observed (PMID: 23361237). The nuclear counter stain is DAPI (blue)
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.Antigen retrieval: Heated Tris-EDTA (pH9.0) (ab94681).
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![Anti-DPP4 antibody [EPR18215] (ab187048)](https://www.abcam.com/ps/products/187/ab187048/Images/ab187048-6-benefits-of-recombinant-antibodies.png)
Anti-DPP4 antibody [EPR18215] (ab187048)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab187048 has been referenced in 1 publication.
- Zhou X et al. DPP4 Inhibitor Attenuates Severe Acute Pancreatitis-Associated Intestinal Inflammation via Nrf2 Signaling. Oxid Med Cell Longev 2019:6181754 (2019). PubMed: 31827684