Recombinant
RabMAb

Recombinant Anti-DPP4 antibody [EPR18215] (ab187048)

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Overview

  • Product name

    Anti-DPP4 antibody [EPR18215]
    See all DPP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR18215] to DPP4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Mouse DPP4 aa 1-250. The exact sequence is proprietary.
    Database link: P28843

  • Positive control

    • WB: Rat lung, thymus, liver and kidney tissue lysate; mouse lung and thymus tissue lysate. IHC-P: Mouse liver tissue; rat liver tissue. IHC-Fr: Mouse liver tissue; rat liver tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab187048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/200 - 1/1000.

Rat: use at 1/200

Mouse: use at 1/1000

Target

  • Function

    Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.

  • Tissue specificity

    Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.

  • Sequence similarities

    Belongs to the peptidase S9B family. DPPIV subfamily.

  • Domain

    The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.

  • Post-translational
    modifications

    The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
    N- and O-Glycosylated.
    Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.

  • Cellular localization

    Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD26 antigen antibody
      • ADA-binding protein antibody
      • ADABP antibody
      • ADCP 2 antibody
      • ADCP-2 antibody
      • ADCP2 antibody
      • Adenosine deaminase complexing protein 2 antibody
      • CD 26 antibody
      • CD26 antibody
      • CD26 antigen 3 antibody
      • Dipeptidyl peptidase 4 antibody
      • Dipeptidyl peptidase 4 soluble form antibody
      • Dipeptidyl peptidase IV antibody
      • Dipeptidyl peptidase IV membrane form antibody
      • Dipeptidyl peptidase IV soluble form antibody
      • Dipeptidyl peptidase, intestinal antibody
      • Dipeptidylpeptidase 4 antibody
      • Dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2) antibody
      • Dipeptidylpeptidase IV antibody
      • DPP 4 antibody
      • DPP IV antibody
      • DPP IV estoenzyme antibody
      • DPP4 antibody
      • DPP4_HUMAN antibody
      • DPPIV antibody
      • Intestinal dipeptidyl peptidase antibody
      • T cell activation antigen CD26 antibody
      • T-cell activation antigen CD26 antibody
      • TP 103 antibody
      • TP103 antibody
      see all

    Images

    • Western blot - Anti-DPP4 antibody [EPR18215] (ab187048)
      Western blot - Anti-DPP4 antibody [EPR18215] (ab187048)
      All lanes : Anti-DPP4 antibody [EPR18215] (ab187048) at 1/1000 dilution

      Lane 1 : Mouse lung lysate
      Lane 2 : Rat lung lysate
      Lane 3 : Mouse thymus lysate
      Lane 4 : Rat thymus lysate
      Lane 5 : Rat liver lysate
      Lane 6 : Rat kidney lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

      Predicted band size: 88 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST 

      Exposure times.

      Lanes 1, 3, 4: 30 seconds

      Lane 2: 20 seconds

      Lane 5: 10 seconds

      Lane 6: 15 seconds

      The molecular weight observed is consistent with what has been described in the literature (PMID: 25589660).

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (ab187048)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (ab187048)

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD26 with ab187048 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on bile canaliculi of mouse liver is observed (PMID: 23361237). Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (ab187048)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPP4 antibody [EPR18215] (ab187048)

      Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling CD26 with ab187048 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on bile canaliculi of rat liver is observed (PMID: 23361237). Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (ab187048)
      Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (ab187048)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized mouse liver tissue labeling CD26 with ab187048 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Strong positive staining on the bile canaliculi and moderate positive staining in sinusoid endothelial cells is observed (PMID: 23361237). The nuclear counter stain is DAPI (blue)
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      Antigen retrieval: Heated Tris-EDTA (pH9.0) (ab94681).

    • Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (ab187048)
      Immunohistochemistry (Frozen sections) - Anti-DPP4 antibody [EPR18215] (ab187048)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized rat liver tissue labeling CD26 with ab187048 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Strong positive staining on the bile canaliculi and moderate positive staining in sinusoid endothelial cells is observed (PMID: 23361237). The nuclear counter stain is DAPI (blue)
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      Antigen retrieval: Heated Tris-EDTA (pH9.0) (ab94681).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References

    Publishing research using ab187048? Please let us know so that we can cite the reference in this datasheet.

    ab187048 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DPP4 antibody [EPR18215]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Nov 15 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DPP4 antibody [EPR18215]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Nov 15 2018

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