Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20819] to DPP4
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-DPP4 antibody [EPR20819]
See all DPP4 primary antibodies
DescriptionRabbit monoclonal [EPR20819] to DPP4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human DPP4 aa 100-500 (Cysteine residue). The exact sequence is proprietary.
Database link: P27487
- WB: Caco-2 and LoVo whole cell lysate. Human fetal kidney, fetal lung and fetal liver lysate. IHC-P: Human liver tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab215711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).|
|IHC-P||1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
Tissue specificityExpressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
Sequence similaritiesBelongs to the peptidase S9B family. DPPIV subfamily.
DomainThe extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.
modificationsThe soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
N- and O-Glycosylated.
Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.
Cellular localizationCell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.
- Information by UniProt
- CD26 antigen antibody
- ADA-binding protein antibody
- ADABP antibody
All lanes : Anti-DPP4 antibody [EPR20819] (ab215711) at 0.4 µg/ml
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate prepared using RIPA lysis method
Lane 2 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate prepared using 1%SDS hot lysis method
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 88 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
For this product, we recommend 1%SDS hot lysis method.
For lysate preparation protocol, please refer to the protocol book in the protocol section or here (downloadable copy).
Lane 1: 3 minutes
Lane 2: 7 seconds
All lanes : Anti-DPP4 antibody [EPR20819] (ab215711) at 1/1000 dilution
Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : LoVo (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg
Lane 3 : Human fetal kidney at 10 µg
Lane 4 : Human fetal lung at 10 µg
Lane 5 : Human fetal liver at 10 µg
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lanes 3-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 88 kDa
Blocking: 5% NFDM/TBST.
Lanes 1,3: 3 minutes
Lane 2: 15 seconds
Lane 4: 1 minute
Lane 5: 30 seconds
The molecular weight observed is consistent with what has been described in the literature (PMID: 25589660).
All the lysates were preparaed using 1%SDS hot lysis method
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD26 with ab215711 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on the bile canaliculi of human liver (PMID: 23361237) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab215711 has been referenced in 1 publication.
- Lindgren O et al. Gliptin-associated Bullous Pemphigoid and the Expression of Dipeptidyl Peptidase-4/CD26 in Bullous Pemphigoid. Acta Derm Venereol 99:602-609 (2019). PubMed: 30848289