Anti-DPP6 antibody - Catalytic domain (ab41811)
- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-DPP6 antibody - Catalytic domain
See all DPP6 primary antibodies -
DescriptionRabbit polyclonal to DPP6 - Catalytic domain
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Host speciesRabbit
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SpecificityThis antibody will recognize the three longest forms of DPP6 but not the 353 amino acid form. It will not recognize the other family members.
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Tested applicationsSuitable for: IHC-Fr, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human, Zebrafish
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Immunogen
Synthetic peptide based on the catalytic domain of human DPP6.
(Peptide available as ab44770.)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 50% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab41811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | ||
| WB |
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 98 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceDPP6 (DPPX, also known as potassium channel accelerating factor Dppx) is a Type-II serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa -Gly orientation. DPP6 has an Asp instead of Ser in the catalytic site. DPP6 is a member of a broader family of dipeptidyl peptidases including DPP4, FAP/Seprase, DPP2, DPP8, DPP9, DPP10, which have differing substrate specificity and tissue localizations. The surface-bound DPP6 is a homodimer, and cleavage of in the stalk region releases a shed form of DPP6. The shed is the form found in serum. DPP6 has been found in highest abundance in the brain, but also in the kidney, liver and lung.
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Cellular localizationCell Membrane; single-pass type II membrane protein
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Database links
- Entrez Gene: 1804 Human
- Entrez Gene: 13483 Mouse
- Entrez Gene: 29272 Rat
- Omim: 126141 Human
- SwissProt: P42658 Human
- SwissProt: Q9Z218 Mouse
- SwissProt: P46101 Rat
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Alternative names
- Dipeptidyl aminopeptidase IV related protein antibody
- Dipeptidyl aminopeptidase like protein 6 antibody
- Dipeptidyl aminopeptidase related protein antibody
see all
Images
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Western blot - Anti-DPP6 antibody - Catalytic domain (ab41811)All lanes : Anti-DPP6 antibody - Catalytic domain (ab41811) at 1/1000 dilution
Lane 1 : Mouse Brain
Lane 2 : DU145 / IL1-beta Cell media
Predicted band size: 98 kDa
Observed band size: 105 kDa (why is the actual band size different from the predicted?)
Additional bands at: 60 kDa (possible truncated form) -
Immunohistochemistry (Frozen sections) - Anti-DPP6 antibody - Catalytic domain (ab41811)Image courtesy of Dr Brent Bill by Abreview.ab41811 staining DPP6 - Catalytic domain in zebrafish brain tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in paraformaldehyde, blocked with 2% serum for 2 hours at 24°C and then incubated with ab41811 at a 1/25 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.
Protocols
References
This product has been referenced in:
- Lin L et al. DPP6 domains responsible for its localization and function. J Biol Chem 289:32153-65 (2014). WB, IP . Read more (PubMed: 25190807) »
- Nadin BM & Pfaffinger PJ A new TASK for Dipeptidyl Peptidase-like Protein 6. PLoS One 8:e60831 (2013). WB ; Rat . Read more (PubMed: 23593319) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"