Key features and details
- Rabbit polyclonal to DPP6 - Catalytic domain
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DPP6 antibody - Catalytic domain
See all DPP6 primary antibodies
DescriptionRabbit polyclonal to DPP6 - Catalytic domain
SpecificityThis antibody will recognize the three longest forms of DPP6 but not the 353 amino acid form. It will not recognize the other family members.
Tested Applications & Species
Application Species WBMouse
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
RelevanceDPP6 (DPPX, also known as potassium channel accelerating factor Dppx) is a Type-II serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa -Gly orientation. DPP6 has an Asp instead of Ser in the catalytic site. DPP6 is a member of a broader family of dipeptidyl peptidases including DPP4, FAP/Seprase, DPP2, DPP8, DPP9, DPP10, which have differing substrate specificity and tissue localizations. The surface-bound DPP6 is a homodimer, and cleavage of in the stalk region releases a shed form of DPP6. The shed is the form found in serum. DPP6 has been found in highest abundance in the brain, but also in the kidney, liver and lung.
Cellular localizationCell Membrane; single-pass type II membrane protein
- Dipeptidyl aminopeptidase IV related protein antibody
- Dipeptidyl aminopeptidase like protein 6 antibody
- Dipeptidyl aminopeptidase related protein antibody
All lanes : Anti-DPP6 antibody - Catalytic domain (ab41811) at 1/1000 dilution
Lane 1 : Mouse Brain
Lane 2 : DU145 / IL1-beta Cell media
Predicted band size: 98 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa (possible truncated form)
ab41811 has been referenced in 6 publications.
- Tabor GT et al. A novel bungarotoxin binding site-tagged construct reveals MAPK-dependent Kv4.2 trafficking. Mol Cell Neurosci 98:121-130 (2019). PubMed: 31212013
- Lin L et al. DPP6 domains responsible for its localization and function. J Biol Chem 289:32153-65 (2014). WB, IP . PubMed: 25190807
- Nadin BM & Pfaffinger PJ A new TASK for Dipeptidyl Peptidase-like Protein 6. PLoS One 8:e60831 (2013). WB ; Rat . PubMed: 23593319
- Lin L et al. DPP6 regulation of dendritic morphogenesis impacts hippocampal synaptic development. Nat Commun 4:2270 (2013). PubMed: 23912628
- Nadin BM & Pfaffinger PJ Dipeptidyl peptidase-like protein 6 is required for normal electrophysiological properties of cerebellar granule cells. J Neurosci 30:8551-65 (2010). PubMed: 20573902
- Jerng HH & Pfaffinger PJ Multiple Kv channel-interacting proteins contain an N-terminal transmembrane domain that regulates Kv4 channel trafficking and gating. J Biol Chem 283:36046-59 (2008). WB ; Human . PubMed: 18957440