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    dpp6-antibody-catalytic-domain-ab41811.pdf

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Anti-DPP6 antibody - Catalytic domain (ab41811)

  • Datasheet
  • SDS
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Western blot - Anti-DPP6 antibody - Catalytic domain (ab41811)

    Key features and details

    • Rabbit polyclonal to DPP6 - Catalytic domain
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-DPP6 antibody - Catalytic domain
      See all DPP6 primary antibodies
    • Description

      Rabbit polyclonal to DPP6 - Catalytic domain
    • Host species

      Rabbit
    • Specificity

      This antibody will recognize the three longest forms of DPP6 but not the 353 amino acid form. It will not recognize the other family members.
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data
    • Immunogen

      Synthetic peptide based on the catalytic domain of human DPP6.

      Read Abcam's proprietary immunogen policy (Peptide available as ab44770.)
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituent: 50% Glycerol
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • Other Enzymes

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab41811 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    Application Abreviews Notes
    WB (1)
    Notes
  • Application notes
    WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 98 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      DPP6 (DPPX, also known as potassium channel accelerating factor Dppx) is a Type-II serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa -Gly orientation. DPP6 has an Asp instead of Ser in the catalytic site. DPP6 is a member of a broader family of dipeptidyl peptidases including DPP4, FAP/Seprase, DPP2, DPP8, DPP9, DPP10, which have differing substrate specificity and tissue localizations. The surface-bound DPP6 is a homodimer, and cleavage of in the stalk region releases a shed form of DPP6. The shed is the form found in serum. DPP6 has been found in highest abundance in the brain, but also in the kidney, liver and lung.
    • Cellular localization

      Cell Membrane; single-pass type II membrane protein
    • Database links

      • Entrez Gene: 1804 Human
      • Entrez Gene: 13483 Mouse
      • Omim: 126141 Human
      • SwissProt: P42658 Human
      • SwissProt: Q9Z218 Mouse
      • Alternative names

        • Dipeptidyl aminopeptidase IV related protein antibody
        • Dipeptidyl aminopeptidase like protein 6 antibody
        • Dipeptidyl aminopeptidase related protein antibody
        • Dipeptidyl peptidase 6 antibody
        • Dipeptidyl peptidase IV like protein antibody
        • Dipeptidyl peptidase IV related protein antibody
        • Dipeptidylpeptidase 6 antibody
        • Dipeptidylpeptidase VI antibody
        • DPP VI antibody
        • DPPX antibody
        • VF2 antibody
        see all

      Images

      • Western blot - Anti-DPP6 antibody - Catalytic domain (ab41811)
        Western blot - Anti-DPP6 antibody - Catalytic domain (ab41811)
        All lanes : Anti-DPP6 antibody - Catalytic domain (ab41811) at 1/1000 dilution

        Lane 1 : Mouse Brain
        Lane 2 : DU145 / IL1-beta Cell media

        Predicted band size: 98 kDa
        Observed band size: 105 kDa why is the actual band size different from the predicted?
        Additional bands at: 60 kDa (possible truncated form)

      Protocols

      • Recommended WB protocol

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (6)

      Publishing research using ab41811? Please let us know so that we can cite the reference in this datasheet.

      ab41811 has been referenced in 6 publications.

      • Tabor GT  et al. A novel bungarotoxin binding site-tagged construct reveals MAPK-dependent Kv4.2 trafficking. Mol Cell Neurosci 98:121-130 (2019). PubMed: 31212013
      • Lin L  et al. DPP6 domains responsible for its localization and function. J Biol Chem 289:32153-65 (2014). WB, IP . PubMed: 25190807
      • Nadin BM & Pfaffinger PJ A new TASK for Dipeptidyl Peptidase-like Protein 6. PLoS One 8:e60831 (2013). WB ; Rat . PubMed: 23593319
      • Lin L  et al. DPP6 regulation of dendritic morphogenesis impacts hippocampal synaptic development. Nat Commun 4:2270 (2013). PubMed: 23912628
      • Nadin BM & Pfaffinger PJ Dipeptidyl peptidase-like protein 6 is required for normal electrophysiological properties of cerebellar granule cells. J Neurosci 30:8551-65 (2010). PubMed: 20573902
      • Jerng HH & Pfaffinger PJ Multiple Kv channel-interacting proteins contain an N-terminal transmembrane domain that regulates Kv4 channel trafficking and gating. J Biol Chem 283:36046-59 (2008). WB ; Human . PubMed: 18957440

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-DPP6 antibody - Catalytic domain

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Zebrafish Tissue lysate - whole (Neuronal tissue)
      Gel Running Conditions
      Reduced Denaturing (7.5%)
      Treatment
      RIPA buffer with protease inhibitor
      Specification
      Neuronal tissue
      Blocking step
      Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted May 24 2016

      Immunohistochemistry (Frozen sections) abreview for Anti-DPP6 antibody - Catalytic domain

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Zebrafish Tissue sections (Brain)
      Specification
      Brain
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
      Read More

      Dr. Brent Bill

      Verified customer

      Submitted Sep 22 2011

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