Product nameAnti-DPP6 antibody - Catalytic domain
See all DPP6 primary antibodies
DescriptionRabbit polyclonal to DPP6 - Catalytic domain
SpecificityThis antibody will recognize the three longest forms of DPP6 but not the 353 amino acid form. It will not recognize the other family members.
Tested applicationsSuitable for: IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Zebrafish
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab41811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceDPP6 (DPPX, also known as potassium channel accelerating factor Dppx) is a Type-II serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa -Gly orientation. DPP6 has an Asp instead of Ser in the catalytic site. DPP6 is a member of a broader family of dipeptidyl peptidases including DPP4, FAP/Seprase, DPP2, DPP8, DPP9, DPP10, which have differing substrate specificity and tissue localizations. The surface-bound DPP6 is a homodimer, and cleavage of in the stalk region releases a shed form of DPP6. The shed is the form found in serum. DPP6 has been found in highest abundance in the brain, but also in the kidney, liver and lung.
Cellular localizationCell Membrane; single-pass type II membrane protein
- Dipeptidyl aminopeptidase IV related protein antibody
- Dipeptidyl aminopeptidase like protein 6 antibody
- Dipeptidyl aminopeptidase related protein antibody
All lanes : Anti-DPP6 antibody - Catalytic domain (ab41811) at 1/1000 dilution
Lane 1 : Mouse Brain
Lane 2 : DU145 / IL1-beta Cell media
Predicted band size: 98 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa (possible truncated form)
ab41811 staining DPP6 - Catalytic domain in zebrafish brain tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in paraformaldehyde, blocked with 2% serum for 2 hours at 24°C and then incubated with ab41811 at a 1/25 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.
This product has been referenced in:
- Tabor GT et al. A novel bungarotoxin binding site-tagged construct reveals MAPK-dependent Kv4.2 trafficking. Mol Cell Neurosci 98:121-130 (2019). Read more (PubMed: 31212013) »
- Lin L et al. DPP6 domains responsible for its localization and function. J Biol Chem 289:32153-65 (2014). WB, IP . Read more (PubMed: 25190807) »