Overview

  • Product name
    Anti-DPP8 antibody
    See all DPP8 primary antibodies
  • Description
    Rabbit polyclonal to DPP8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1-39 (MAAAMETEQL GVEIFETADC EENIESQDRP KLEPFYVER) of DPP8 (NP_060213; UniProt ID: Q6V1X1 isoform 4).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • WB: A431 whole cell lysate; 293T, H1299 and HepG2 cell lines ICC/IF: HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 10% Glycerol, 1.21% Tris
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: for isoform 4, 90 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. May play a role in T-cell activation and immune function.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in testis, placenta, prostate, muscle and brain.
  • Sequence similarities
    Belongs to the peptidase S9B family. DPPIV subfamily.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • Dipeptidyl peptidase 8 antibody
      • Dipeptidyl peptidase IV-related protein 1 antibody
      • Dipeptidyl peptidase VIII antibody
      • Dipeptidylpeptidase 8 antibody
      • DP8 antibody
      • DPP VIII antibody
      • Dpp8 antibody
      • DPP8_HUMAN antibody
      • DPRP-1 antibody
      • DPRP1 antibody
      • EC 3.4.14.5 antibody
      • MSTP141 antibody
      • Prolyl dipeptidase DPP8 antibody
      see all

    Images

    • Western blot - Anti-DPP8 antibody (ab96470)
      Western blot - Anti-DPP8 antibody (ab96470)
      Anti-DPP8 antibody (ab96470) at 1/1000 dilution + A431 whole cell lysate at 30 µg

      Predicted band size: for isoform 4, 90 kDa



      7.5% SDS-PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-DPP8 antibody (ab96470)
      Immunocytochemistry/ Immunofluorescence - Anti-DPP8 antibody (ab96470)
      ab96470, at a 1/200 dilution, staining DPP8 in paraformaldehyde fixed HeLa cells by Immunofluorescence.
      Lower image is merged with DNA probe.
    • Western blot - Anti-DPP8 antibody (ab96470)
      Western blot - Anti-DPP8 antibody (ab96470)

    References

    ab96470 has not yet been referenced specifically in any publications.

    Publishing research using ab96470? Please let us know so that we can cite the reference in this datasheet.

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