Anti-DPP8 antibody (ab96470)
Key features and details
- Rabbit polyclonal to DPP8
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DPP8 antibody
See all DPP8 primary antibodies -
Description
Rabbit polyclonal to DPP8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region within amino acids 1-39 (MAAAMETEQL GVEIFETADC EENIESQDRP KLEPFYVER) of DPP8 (NP_060213; UniProt ID: Q6V1X1 isoform 4).
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Positive control
- WB: A431 whole cell lysate; 293T, H1299 and HepG2 cell lines ICC/IF: HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: for isoform 4, 90 kDa.
|
|
| ICC/IF |
1/100 - 1/200.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: for isoform 4, 90 kDa. |
|
ICC/IF
1/100 - 1/200. |
Target
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Function
Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. May play a role in T-cell activation and immune function. -
Tissue specificity
Ubiquitously expressed, with highest levels in testis, placenta, prostate, muscle and brain. -
Sequence similarities
Belongs to the peptidase S9B family. DPPIV subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 54878 Human
- Omim: 606819 Human
- SwissProt: Q6V1X1 Human
- Unigene: 591106 Human
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Alternative names
- Dipeptidyl peptidase 8 antibody
- Dipeptidyl peptidase IV-related protein 1 antibody
- Dipeptidyl peptidase VIII antibody
see all
Images
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Western blot - Anti-DPP8 antibody (ab96470)Anti-DPP8 antibody (ab96470) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: for isoform 4, 90 kDa
7.5% SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-DPP8 antibody (ab96470)ab96470, at a 1/200 dilution, staining DPP8 in paraformaldehyde fixed HeLa cells by Immunofluorescence.
Lower image is merged with DNA probe. -
Western blot - Anti-DPP8 antibody (ab96470)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab96470 has not yet been referenced specifically in any publications.