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    dpp8-antibody-ab96470.pdf

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Cell Biology Proteolysis / Ubiquitin Proteolytic enzymes Serine protease DPPs
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Anti-DPP8 antibody (ab96470)

  • Datasheet
  • SDS
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Western blot - Anti-DPP8 antibody (ab96470)
  • Immunocytochemistry/ Immunofluorescence - Anti-DPP8 antibody (ab96470)
  • Western blot - Anti-DPP8 antibody (ab96470)

Key features and details

  • Rabbit polyclonal to DPP8
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant human DPP8 protein (ab79662)

View more associated products

Overview

  • Product name

    Anti-DPP8 antibody
    See all DPP8 primary antibodies
  • Description

    Rabbit polyclonal to DPP8
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1-39 (MAAAMETEQL GVEIFETADC EENIESQDRP KLEPFYVER) of DPP8 (NP_060213; UniProt ID: Q6V1X1 isoform 4).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: A431 whole cell lysate; 293T, H1299 and HepG2 cell lines ICC/IF: HeLa cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Serine protease
    • DPPs

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Hep G2 cytoplasmic extract lysate (ab14659)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant human DPP8 protein (ab79662)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab96470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: for isoform 4, 90 kDa.
ICC/IF
1/100 - 1/200.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: for isoform 4, 90 kDa.
ICC/IF
1/100 - 1/200.

Target

  • Function

    Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. May play a role in T-cell activation and immune function.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in testis, placenta, prostate, muscle and brain.
  • Sequence similarities

    Belongs to the peptidase S9B family. DPPIV subfamily.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q6V1X1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 54878 Human
    • Omim: 606819 Human
    • SwissProt: Q6V1X1 Human
    • Unigene: 591106 Human
    • Alternative names

      • Dipeptidyl peptidase 8 antibody
      • Dipeptidyl peptidase IV-related protein 1 antibody
      • Dipeptidyl peptidase VIII antibody
      • Dipeptidylpeptidase 8 antibody
      • DP8 antibody
      • DPP VIII antibody
      • Dpp8 antibody
      • DPP8_HUMAN antibody
      • DPRP-1 antibody
      • DPRP1 antibody
      • EC 3.4.14.5 antibody
      • MSTP141 antibody
      • Prolyl dipeptidase DPP8 antibody
      see all

    Images

    • Western blot - Anti-DPP8 antibody (ab96470)
      Western blot - Anti-DPP8 antibody (ab96470)
      Anti-DPP8 antibody (ab96470) at 1/1000 dilution + A431 whole cell lysate at 30 µg

      Predicted band size: for isoform 4, 90 kDa



      7.5% SDS-PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-DPP8 antibody (ab96470)
      Immunocytochemistry/ Immunofluorescence - Anti-DPP8 antibody (ab96470)
      ab96470, at a 1/200 dilution, staining DPP8 in paraformaldehyde fixed HeLa cells by Immunofluorescence.
      Lower image is merged with DNA probe.
    • Western blot - Anti-DPP8 antibody (ab96470)
      Western blot - Anti-DPP8 antibody (ab96470)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab96470? Please let us know so that we can cite the reference in this datasheet.

    ab96470 has not yet been referenced specifically in any publications.

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