Western blot abreview for Anti-DPP9 antibody - Catalytic domain

Excellent
Abreviews
Application
Western blot
Sample
Cow Purified protein (testes)
Loading amount
0.32 µg
Specification
testes
Gel Running Conditions
Reduced Denaturing (7,5% gel)
Blocking step
0.05 M Tris buffer containing 0.15 M NaCl, 5 mM NaN3 and 2% Tween 20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.05 M Tris, 0.15 M NaCl, 0.1% Tween 20

Secondary antibody

Name
Non-Abcam antibody was used: HRP-conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 106 kDa
Positive control
lane 5: bovine DPP9
Negative control
lane 1: molecular weight standards
lane 2: human DPPII
lane 3: human DPPIV
lane 4: recombinant DPP8
lane 6: molecular weight standards

Additional data

PubMed ID
19188489: View
Additional Notes
nitrocellulose membrane
primary antibody was incubated for 1 hr at room temperature and overnight at 4°C

Dr. Veronique Dubois

Verified customer

Submitted Jun 16 2009

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