Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DPP9 antibody - Catalytic domain

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (testis)
Specification
testis
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer pH 9.0
Permeabilization
No
Blocking step
3% H2O2 in TBS as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS, 0.3% Triton X-100,1 % BSA

Secondary antibody

Name
Non-Abcam antibody was used: biotinylated polyclonal goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

PubMed ID
19188489: View
Additional Notes
The sections were treated for 10 min with 3% H2O2 in TBS to block endogenous peroxidase activity and incubated subsequently for 30 min with 10% nonimmune goat serum to minimize nonspecific antibody binding
Sections were incubated with the primary polyclonal antibody for 1 hr at room temperature followed by incubating overnight at 4°C

Dr. Veronique Dubois

Verified customer

Submitted Jun 11 2009

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