Western blot abreview for Anti-DPP9 antibody - Catalytic domain

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
5 µg
Specification
HeLa cells
Treatment
siRNA
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS-tween, 5% milk

Secondary antibody

Name
Non-Abcam antibody was used: Biorad
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL, MILLIPORE
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: 120 kDa
Negative control
siRNA treated cells

Additional data

Additional Notes
Does not work in IP

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Submitted Oct 19 2009

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