• Product name

    Anti-Dppa2 antibody
  • Description

    Rabbit polyclonal to Dppa2
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse Dppa2 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab104208)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: IOUD2; F9; Mouse EG; E14Tg2a.



Our Abpromise guarantee covers the use of ab91318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
ICC/IF Use a concentration of 1 µg/ml.


  • Relevance

    Dppa2 is a SAP domain protein expressed only in pluripotent cells and the developing germ line.
  • Cellular localization

  • Database links

  • Alternative names

    • cancer/testis antigen 100 antibody
    • CT100 antibody
    • Developmental Pluripotency Associated 2 antibody
    • developmental pluripotency-associated gene 2 antibody
    • developmental pluripotency-associated protein 2 antibody
    • ECAT15-2 antibody
    • embryonic stem cell (ESC) associated transcript 15-2 antibody
    • PESCRG1 antibody
    • Pluripotent embryonic stem cell related protein 1 antibody
    • Pluripotent embryonic stem cell-related gene 1 antibody
    • Pluripotent embryonic stem cell-related gene 1 protein antibody
    see all


  • All lanes : Anti-Dppa2 antibody (ab91318) at 1 µg/ml

    Lane 1 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
    Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
    Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?

    Exposure time: 3 minutes
  • ICC/IF image of ab91318 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91318, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab91318, DyLight® 488 goat anti-rabbit IgX (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Yan YL  et al. DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program. PLoS Biol 17:e3000324 (2019). Read more (PubMed: 31226106) »
  • Tung PY  et al. Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes. Stem Cells N/A:N/A (2013). WB, IP, ChIP . Read more (PubMed: 23963736) »
See all 2 Publications for this product

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