Product nameAnti-Dppa2 antibody
DescriptionRabbit polyclonal to Dppa2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse Dppa2 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: IOUD2; F9; Mouse EG; E14Tg2a.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab91318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
RelevanceDppa2 is a SAP domain protein expressed only in pluripotent cells and the developing germ line.
- cancer/testis antigen 100 antibody
- CT100 antibody
- Developmental Pluripotency Associated 2 antibody
All lanes : Anti-Dppa2 antibody (ab91318) at 1 µg/ml
Lane 1 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ICC/IF image of ab91318 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91318, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab91318, DyLight® 488 goat anti-rabbit IgX (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Yan YL et al. DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program. PLoS Biol 17:e3000324 (2019). Read more (PubMed: 31226106) »
- Tung PY et al. Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes. Stem Cells N/A:N/A (2013). WB, IP, ChIP . Read more (PubMed: 23963736) »