Key features and details
- Rabbit polyclonal to Dppa2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Dppa2 antibody
DescriptionRabbit polyclonal to Dppa2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse Dppa2 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: IOUD2; F9; Mouse EG; E14Tg2a.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab91318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
RelevanceDppa2 is a SAP domain protein expressed only in pluripotent cells and the developing germ line.
- cancer/testis antigen 100 antibody
- CT100 antibody
- Developmental Pluripotency Associated 2 antibody
All lanes : Anti-Dppa2 antibody (ab91318) at 1 µg/ml
Lane 1 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lane 3 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ICC/IF image of ab91318 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91318, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab91318, DyLight® 488 goat anti-rabbit IgX (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab91318 has been referenced in 2 publications.
- Yan YL et al. DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program. PLoS Biol 17:e3000324 (2019). PubMed: 31226106
- Tung PY et al. Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes. Stem Cells N/A:N/A (2013). WB, IP, ChIP . PubMed: 23963736