Key features and details
- Rabbit polyclonal to Dppa4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dppa4 antibody
See all Dppa4 primary antibodies
DescriptionRabbit polyclonal to Dppa4
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to a region within amino acids 30-304 of Human Dppa4 (UniProt ID:Q7L190).
- NT2D1 and Human ESC whole cell lysate. Human ESC cells. H661 xenograft.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 34 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.|
|ICC/IF||1/100 - 1/1000.|
FunctionMay be involved in the maintenance of active epigenetic status of target genes (By similarity). May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
- Information by UniProt
- 2410091M23Rik antibody
- Developmental pluripotency associated 4 antibody
- Developmental pluripotency associated protein 4 antibody
Immunohistochemical analysis of paraffin embedded H661 (human cell line) xenograft tissue labelling Dppa4 with ab154642 at a 1/100 dilution.
Anti-Dppa4 antibody (ab154642) at 1/500 dilution + NT2D1 whole cell lysate at 30 µg/ml
Predicted band size: 34 kDa
12% SDS PAGE
Anti-Dppa4 antibody (ab154642) at 1/500 dilution + Human ESC whole cell lysate at 20 µg
Predicted band size: 34 kDa
12% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed Human ESC labeling Dppa4 with ab154642 at a 1/400 dilution.
The lower image has been stained with Hoechst 33342.
ab154642 has been referenced in 2 publications.
- Nakai R et al. Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata). Sci Rep 8:12187 (2018). PubMed: 30111816
- Yu-Ching Lin Z et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev Biol N/A:N/A (2015). IHC-P ; Common marmoset . PubMed: 25624265