Key features and details
- Rabbit polyclonal to Dppa4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dppa4 antibody
See all Dppa4 primary antibodies
DescriptionRabbit polyclonal to Dppa4
SpecificityThe immunogen was designed to raise an antibody against mouse Dppa4. However, the antibody does not recognise a band of the expected size of Dppa4 in the mouse cell lysate tested. This may be because Dppa4 is not expressed in sufficient amounts for detection by Western Blotting in those cells. The immunogen also contained a sequence of 12 amino acids found in human Dppa4. The antibody recognises a band of approximately the expected size of Dppa4 in human embryonic stem cell lysate. Additionally, the immuogen contains a sequence of 12 consecutive amino acids which are found in human Dppa2 and a sequence of 13 amino acids of which 11 are found in mouse Dppa2. Therefore, it cannot be ruled out that the antibody recognizes Dppa2.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Does not react with: Mouse
Synthetic peptide corresponding to Mouse Dppa4 aa 200 to the C-terminus.
(Peptide available as
- Human Embryonic Stem Cell Lysate; Mouse Embryonic Stem Cell Lysate;
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab31648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).|
FunctionMay be involved in the maintenance of active epigenetic status of target genes (By similarity). May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
- Information by UniProt
- 2410091M23Rik antibody
- Developmental pluripotency associated 4 antibody
- Developmental pluripotency associated protein 4 antibody
All lanes : Anti-Dppa4 antibody (ab31648) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Predicted band size: 33 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa (possible IgG), 48 kDa (possible glycosylated form), 48 kDa (possible non-specific binding)
ab31648 recognized a band of approximately the predicted molecular weight (34 kDa) of Dppa4 in human, but not in mouse, embryonic stem cell lysates. The identity of the ~ 50 kDa band is not known.
ab31648 has been referenced in 1 publication.
- Takahashi K et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861-72 (2007). WB ; Human . PubMed: 18035408