• Product name

  • Description

    Rabbit polyclonal to Dppa4
  • Host species

  • Specificity

    The immunogen was designed to raise an antibody against mouse Dppa4. However, the antibody does not recognise a band of the expected size of Dppa4 in the mouse cell lysate tested. This may be because Dppa4 is not expressed in sufficient amounts for detection by Western Blotting in those cells. The immunogen also contained a sequence of 12 amino acids found in human Dppa4. The antibody recognises a band of approximately the expected size of Dppa4 in human embryonic stem cell lysate. Additionally, the immuogen contains a sequence of 12 consecutive amino acids which are found in human Dppa2 and a sequence of 13 amino acids of which 11 are found in mouse Dppa2. Therefore, it cannot be ruled out that the antibody recognizes Dppa2.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse Dppa4 aa 200 to the C-terminus.
    (Peptide available as ab31647)

  • Positive control

    • Human Embryonic Stem Cell Lysate; Mouse Embryonic Stem Cell Lysate;


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab31648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).


  • Function

    May be involved in the maintenance of active epigenetic status of target genes (By similarity). May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • 2410091M23Rik antibody
    • Developmental pluripotency associated 4 antibody
    • Developmental pluripotency associated protein 4 antibody
    • Developmental pluripotency-associated protein 4 antibody
    • Dppa 4 antibody
    • DPPA4 antibody
    • DPPA4_HUMAN antibody
    • FLJ10713 antibody
    see all


  • All lanes : Anti-Dppa4 antibody (ab31648) at 1 µg/ml

    Lane 1 : Human Embryonic Stem Cell Lysate
    Lane 2 : Mouse Embryonic Stem Cell Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Predicted band size: 33 kDa
    Observed band size: 36 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 48 kDa (possible IgG), 48 kDa (possible glycosylated form), 48 kDa (possible non-specific binding)

    ab31648 recognized a band of approximately the predicted molecular weight (34 kDa) of Dppa4 in human, but not in mouse, embryonic stem cell lysates. The identity of the ~ 50 kDa band is not known.


This product has been referenced in:

  • Takahashi K  et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861-72 (2007). WB ; Human . Read more (PubMed: 18035408) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab31648.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up