Key features and details
- Mouse polyclonal to Dppa4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dppa4 antibody
See all Dppa4 primary antibodies
DescriptionMouse polyclonal to Dppa4
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length Human Dppa4 (AAH32846.2).
- Human kidney tissue lysate, Dppa4 transfected 293T cell lysate, HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab88586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionMay be involved in the maintenance of active epigenetic status of target genes (By similarity). May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
- Information by UniProt
- 2410091M23Rik antibody
- Developmental pluripotency associated 4 antibody
- Developmental pluripotency associated protein 4 antibody
Anti-Dppa4 antibody (ab88586) at 1 µg/ml + human kidney tissue lysate at 50 µg
Predicted band size: 34 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
All lanes : Anti-Dppa4 antibody (ab88586) at 1 µg/ml
Lane 1 : Dppa4 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
ab88586 at 10µg/ml staining Dppa4 in HeLa cells by Immunofluorescence.
ab88586 has not yet been referenced specifically in any publications.