Anti-Dppa5 antibody (ab236611)
Key features and details
- Rabbit polyclonal to Dppa5
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dppa5 antibody -
Description
Rabbit polyclonal to Dppa5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Dppa5 aa 1-150.
Database link: A6NC42 -
Positive control
- IHC-P: Human liver cancer and ovarian cancer tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/200 - 1/500. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Involved in the maintenance of embryonic stem (ES) cell pluripotency. Dispensable for self-renewal of pluripotent ES cells and establishment of germ cells. Associates with specific target mRNAs. -
Sequence similarities
Belongs to the KHDC1 family.
Contains 1 KH domain. -
Developmental stage
Expressed in primordial germ (PGC), embryonic germ (EGC) and embryonic stem (ESC) cells. Not detected in embryonic carcinoma (ECC) cells. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 340168 Human
- Omim: 611111 Human
- SwissProt: A6NC42 Human
- Unigene: 125331 Human
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Alternative names
- Developmental pluripotency associated 5 antibody
- Developmental pluripotency-associated 5 protein antibody
- DPPA5 antibody
see all
Images
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Dppa5 using ab236611 at a dilution of 1/66 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). -
Paraffin-embedded human liver cancer tissue stained for Dppa5 using ab236611 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Paraffin-embedded human ovarian cancer tissue stained for Dppa5 using ab236611 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236611 has not yet been referenced specifically in any publications.