Recombinant Anti-DPS1 antibody [EPR20843] (ab216869)
![Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)](https://www.abcam.com/ps/products/216/ab216869/Images/ab216869-306849-anti-dps1-antibody-epr20843-western-blot.jpg "Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20843] to DPS1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-DPS1 antibody [EPR20843]
See all DPS1 primary antibodies -
Description
Rabbit monoclonal [EPR20843] to DPS1 -
Host species
Rabbit -
Specificity
collaborative project -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human DPS1 aa 200-300.
Database link: Q5T2R2 -
Positive control
- WB: MCF7, HeLa, A549, K-562, Jurkat, F9, C6, and RAW 264.7 cell lysate. IP: MCF7 cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20843 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab216869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 46 kDa. | |
| IP | 1/30. |
Target
-
Relevance
DPS1 is an enzyme that elongates the prenyl side-chain of coenzyme Q, or ubiquinone, one of the key elements in the respiratory chain. It catalyzes the formation of all trans-polyprenyl pyrophosphates from isopentyl diphosphate in the assembly of polyisoprenoid side chains, the first step in coenzyme Q biosynthesis. The protein may be peripherally associated with the inner mitochondrial membrane, though no transit peptide has been definitively identified to date. Defects in this gene are a cause of coenzyme Q10 deficiency. There are three named isoforms. -
Cellular localization
Mitochondrial and Plasma membrane -
Database links
- Entrez Gene: 23590 Human
- Entrez Gene: 56075 Mouse
- Omim: 607429 Human
- SwissProt: Q5T2R2 Human
- SwissProt: Q33DR2 Mouse
-
Alternative names
- Coenzyme Q1 homolog antibody
- COQ1 antibody
- Decaprenyl pyrophosphate synthetase subunit 1 antibody
see all
Images
-
Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)All lanes : Anti-DPS1 antibody [EPR20843] (ab216869) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 6 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes.
The band observed likely represents isoforms 2 or 3 based on their predicted molecular masses.
-
![Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)](https://www.abcam.com/ps/products/216/ab216869/Images/ab216869-306850-anti-dps1-antibody-epr20843-western-blot.jpg)
Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Lane 1 : Mouse kidney lysate
Lane 2 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 3 : RAW 264.7(mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 46 kDaBlocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lane 1: 3 minutes
Lane 2-3: 15 seconds.The band observed likely represents isoforms 2 or 3 based on their predicted molecular masses.
-
![Immunoprecipitation - Anti-DPS1 antibody [EPR20843] (ab216869)](https://www.abcam.com/ps/products/216/ab216869/Images/ab216869-306848-anti-dps1-antibody-epr20843-immunoprecipitation.jpg)
Immunoprecipitation - Anti-DPS1 antibody [EPR20843] (ab216869)DPS1 was immunoprecipitated from 10 μg of MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab216869 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216869 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg (input).
Lane 2: ab216869 IP in MCF7 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216869 in MCF7 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 150 seconds. -
![Anti-DPS1 antibody [EPR20843] (ab216869)](https://www.abcam.com/ps/products/216/ab216869/Images/ab216869-4-benefits-of-recombinant-antibodies.png)
Anti-DPS1 antibody [EPR20843] (ab216869)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab216869 has not yet been referenced specifically in any publications.
