Recombinant
RabMAb

Recombinant Anti-DPS1 antibody [EPR20843] (ab216869)

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Overview

  • Product name

    Anti-DPS1 antibody [EPR20843]
    See all DPS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20843] to DPS1

  • Host species

    Rabbit

  • Specificity

    collaborative project

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human DPS1 aa 200-300.
    Database link: Q5T2R2

  • Positive control

    • WB: MCF7, HeLa, A549, K-562, Jurkat, F9, C6, and RAW 264.7 cell lysate. IP: MCF7 cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 46 kDa.
IP 1/30.

Target

  • Relevance

    DPS1 is an enzyme that elongates the prenyl side-chain of coenzyme Q, or ubiquinone, one of the key elements in the respiratory chain. It catalyzes the formation of all trans-polyprenyl pyrophosphates from isopentyl diphosphate in the assembly of polyisoprenoid side chains, the first step in coenzyme Q biosynthesis. The protein may be peripherally associated with the inner mitochondrial membrane, though no transit peptide has been definitively identified to date. Defects in this gene are a cause of coenzyme Q10 deficiency. There are three named isoforms.

  • Cellular localization

    Mitochondrial and Plasma membrane

  • Database links

    • Alternative names

      • Coenzyme Q1 homolog antibody
      • COQ1 antibody
      • Decaprenyl pyrophosphate synthetase subunit 1 antibody
      • DPS antibody
      • hDPS1 antibody
      • Polyprenyl pyrophosphate synthetase antibody
      • Prenyl (decaprenyl) diphosphate synthase, subunit 1 antibody
      • SPS antibody
      • TPRT antibody
      • TPT antibody
      • Trans prenyltransferase antibody
      see all

    Images

    • Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)
      Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)
      All lanes : Anti-DPS1 antibody [EPR20843] (ab216869) at 1/1000 dilution

      Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate
      Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
      Lane 5 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
      Lane 6 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 46 kDa
      Observed band size: 38 kDa
      why is the actual band size different from the predicted?



      Blocking/diluting buffer and concentration: 5% NFDM/TBST 

      Exposure time: 3 minutes.

      The band observed likely represents isoforms 2 or 3 based on their predicted molecular masses.

    • Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)
      Western blot - Anti-DPS1 antibody [EPR20843] (ab216869)
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

      Lane 1 : Mouse kidney lysate
      Lane 2 : C6 (rat glial tumor glial cell) whole cell lysate
      Lane 3 : RAW 264.7(mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 46 kDa



      Blocking/diluting buffer and concentration: 5% NFDM/TBST 

      Exposure time:
      Lane 1: 3 minutes
      Lane 2-3: 15 seconds.

      The band observed likely represents isoforms 2 or 3 based on their predicted molecular masses.

    • Immunoprecipitation - Anti-DPS1 antibody [EPR20843] (ab216869)
      Immunoprecipitation - Anti-DPS1 antibody [EPR20843] (ab216869)

      DPS1 was immunoprecipitated from 10 μg of MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab216869 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216869 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
      Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg (input).
      Lane 2: ab216869 IP in MCF7 whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216869 in MCF7 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST
      Exposure time: 150 seconds.

    References

    ab216869 has not yet been referenced specifically in any publications.

    Publishing research using ab216869? Please let us know so that we can cite the reference in this datasheet.

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