Recombinant
RabMAb

Recombinant Anti-DPS1 antibody [EPR20843] - BSA and Azide free (ab233697)

Overview

  • Product name

    Anti-DPS1 antibody [EPR20843] - BSA and Azide free
    See all DPS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20843] to DPS1 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    collaborative project
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human DPS1 aa 200-300.
    Database link: Q5T2R2

  • Positive control

    • WB: MCF7, HeLa, A549, K-562, Jurkat and F9 cell lysate.
  • General notes

    Ab233697 is the carrier-free version of ab216869. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab233697 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab233697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
IP Use at an assay dependent concentration.

Target

  • Relevance

    DPS1 is an enzyme that elongates the prenyl side-chain of coenzyme Q, or ubiquinone, one of the key elements in the respiratory chain. It catalyzes the formation of all trans-polyprenyl pyrophosphates from isopentyl diphosphate in the assembly of polyisoprenoid side chains, the first step in coenzyme Q biosynthesis. The protein may be peripherally associated with the inner mitochondrial membrane, though no transit peptide has been definitively identified to date. Defects in this gene are a cause of coenzyme Q10 deficiency. There are three named isoforms.
  • Cellular localization

    Mitochondrial and Plasma membrane
  • Database links

  • Alternative names

    • Coenzyme Q1 homolog antibody
    • COQ1 antibody
    • Decaprenyl pyrophosphate synthetase subunit 1 antibody
    • DPS antibody
    • hDPS1 antibody
    • Polyprenyl pyrophosphate synthetase antibody
    • Prenyl (decaprenyl) diphosphate synthase, subunit 1 antibody
    • SPS antibody
    • TPRT antibody
    • TPT antibody
    • Trans prenyltransferase antibody
    see all

Images

  • DPS1 was immunoprecipitated from 10 μg of MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab216869 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216869 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg (input).
    Lane 2: ab216869 IP in MCF7 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216869 in MCF7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 150 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216869).

  • All lanes : Anti-DPS1 antibody [EPR20843] (ab216869) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 5 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
    Lane 6 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa



    Blocking/diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time: 3 minutes.

    The band observed likely represents isoforms 2 or 3 based on their predicted molecular masses.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216869).

References

ab233697 has not yet been referenced specifically in any publications.

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