Key features and details
- Rabbit polyclonal to DPY19L3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DPY19L3 antibody
DescriptionRabbit polyclonal to DPY19L3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- WB: SH-SH5Y and HepG2 whole cell lysate. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab237056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|ICC/IF||1/200 - 1/500.|
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the dpy-19 family.
- Information by UniProt
- D19L3_HUMAN antibody
- DKFZp686J17135 antibody
- Dpy-19-like 3 (C. elegans) antibody
All lanes : Anti-DPY19L3 antibody (ab237056) at 1/500 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for DPY19L3 using ab237056 at a dilution of 1/200 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237056 has not yet been referenced specifically in any publications.