Overview

  • Product name

  • Description

    Rabbit polyclonal to DPY30
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    antigen sequence: YGLTDNVERI VENEKINAEK SSKQKVDLQS LPTRAYLDQT VVPILLQGLA VLAKERPPNP IEFLASYLLK NKAQFEDRN corresponding to a region of Human DPY30.

  • Positive control

    • Human bronchus tissue; U251 MG cells; RT4 and U251 MG cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.

Target

  • Function

    As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. May also play an indirect or direct role in endosomal transport.
  • Sequence similarities

    Belongs to the dpy-30 family.
  • Cellular localization

    Nucleus. Golgi apparatus > trans-Golgi network.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dpy-30-like protein antibody
    • Dpy-30L antibody
    • DPY30 antibody
    • DPY30_HUMAN antibody
    • HDPY-30 antibody
    • Protein dpy-30 homolog antibody
    • Saf19 antibody
    see all

Images

  • ab126352 staining DPY30 in Mouse NIH/3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Tween 20 in PBS and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (2μg/ml in 3% BSA + 0.2% Tween 20 + PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (4μg/ml in blocking solution for 1 hour) was used as the secondary antibody.

    See Abreview

  • ab126352 at a 1/200 dilution staining DPY30 in paraffin embedded human bronchus tissue by Imunohistochemistry.

  • ab126352 at 4 µg/ml staining DPY30 in in PFA/Triton X-100 fixed/permeabilized U251 cells by Immunofluorescence (green).

References

This product has been referenced in:

  • Lu F  et al. Regulation of DNA replication and chromosomal polyploidy by the MLL-WDR5-RBBP5 methyltransferases. Biol Open 5:1449-1460 (2016). Read more (PubMed: 27744293) »
  • Ross NL  et al. Histone-targeted Polyplexes Avoid Endosomal Escape and Enter the Nucleus During Postmitotic Redistribution of ER Membranes. Mol Ther Nucleic Acids 4:e226 (2015). Read more (PubMed: 25668340) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Cell (NIH/3T3 cells)
Specification
NIH/3T3 cells
Permeabilization
Yes - 0.2% PBS-T (PBS with Tween*20)
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 29 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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