The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. May also play an indirect or direct role in endosomal transport.
Detection of DPY30 by Western Blot of Immunoprecipitate. ab187690 at 1µg/ml labeling DPY30 in 293T whole cell lysate immunoprecipitated using ab187690 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes.
Please note: ab187690 has not performed satisfactorily when used for WB of DPY30 in crude preparations (e.g. whole cell lysate). It can be used for WB of enriched DPY30 (e.g. immunoprecipitated).