Key features and details
- Rabbit polyclonal to DPYS
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DPYS antibody
See all DPYS primary antibodies
DescriptionRabbit polyclonal to DPYS
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human kidney tissue; DPYS over-expression HEK293T lysate.
Previously labelled as Dihydropyrimidinase.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionCatalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyzes the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate.
Tissue specificityLiver and kidney.
Involvement in diseaseDefects in DPYS are the cause of dihydropyrimidinase deficiency (DHPD) [MIM:222748]. DHPD is an autosomal recessive disorder characterized by dihydropyrimidinuria and associated with a variable clinical phenotype: epileptic or convulsive attacks, dysmorphic features and severe developmental delay, and congenital microvillous atrophy.
Sequence similaritiesBelongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
modificationsCarbamylation allows a single lysine to coordinate two zinc ions.
- Information by UniProt
- DHP antibody
- DHPase antibody
- Dihydropyrimidinase antibody
All lanes : Anti-DPYS antibody (ab121844) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
Developed using the ECL technique.
Immunofluorescent staining of Human cell line A-431 shows positivity in vesicles and centrosome. Recommended concentration of ab121844 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121844 at 1/500 dilution staining DPYS in Paraffin-embedded Human kidney tissue by Immunohistochemistry.
ab121844 has not yet been referenced specifically in any publications.